Tfap2a Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:165711 Mittal A, et al., Fibronectin and integrin alpha 5 play essential roles in the development of the cardiac neural crest. Mech Dev. 2010 Sep-Dec;127(9-12):472-84
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:5789191
Gene symbol:	Tfap2a
Gene name:	transcription factor AP-2, alpha

Antibody:	Anti-AP-2a (3B5) sc-12726
Detection system:	Secondary antibody coupled to Alexa Fluor 555

Results

1F ©

Specimen 1F: embryonic day 8.5 (more

)
Note: 10 somite stage. Double labeled: red - Tfap2a; green - Fn1 (assay MGI:5789190).

Structure	Level	Pattern	Image	Note
TS13: neural fold	Present		1F

6A ©

Specimen 6A: embryonic day 9.5 (more

)
Note: Double labeled: red - Tfap2a; green - Casp3 (assay MGI:5789193).

Structure	Level	Image
TS15: branchial arch ectoderm	Present	6A
TS15: neural crest	Present	6A
TS15: surface ectoderm	Present	6A

6B ©

Specimen 6B: embryonic day 9.5; Fn1^tm1Hyn/Fn1^tm1Hyn (more

)
Note: Double labeled: red - Tfap2a; green - Casp3 (assay MGI:5789193).

Structure	Level	Image
TS15: branchial arch ectoderm	Present	6B
TS15: neural crest	Present	6B
TS15: surface ectoderm	Present	6B

7E ©

Specimen 7E: embryonic day 8.5 (more

)
Note: Double labeled: red - Tfap2a; green - Casp3 (assay MGI:5789193).

Structure	Level	Pattern	Image	Note
TS13: neural crest	Present		7E
TS13: surface ectoderm	Present		7E

7F ©

7F" ©

7F' ©

Specimen 7F: embryonic day 8.5 (more

)
Note: Double labeled: red - Tfap2a; green - Casp3 (assay MGI:5789193).

Structure	Level	Pattern	Image	Note
TS13: neural crest	Present		7F' , 7F , 7F"
TS13: surface ectoderm	Present		7F' , 7F , 7F"

7G ©

Specimen 7G: embryonic day 8.5 (more

)
Note: Double labeled: red - Tfap2a; green - Casp3 (assay MGI:5789193).

Structure	Level	Pattern	Image	Note
TS13: neural crest	Present		7G
TS13: surface ectoderm	Present		7G

7H ©

Specimen 7H: embryonic day 8.5 (more

)
Note: Double labeled: red - Tfap2a; green - Casp3 (assay MGI:5789193).

Structure	Level	Pattern	Image	Note
TS13: neural crest	Present		7H
TS13: surface ectoderm	Present		7H

7I ©

Specimen 7I: embryonic day 8.5; Fn1^tm1Hyn/Fn1^tm1Hyn (more

)
Note: Double labeled: red - Tfap2a; green - Casp3 (assay MGI:5789193).

Structure	Level	Pattern	Image	Note
TS13: neural crest	Present		7I
TS13: surface ectoderm	Present		7I

7J ©

7J' ©

Specimen 7J: embryonic day 8.5; Fn1^tm1Hyn/Fn1^tm1Hyn (more

)
Note: Double labeled: red - Tfap2a; green - Casp3 (assay MGI:5789193).

Structure	Level	Pattern	Image	Note
TS13: neural crest	Present		7J , 7J'
TS13: surface ectoderm	Present		7J , 7J'

7K ©

Specimen 7K: embryonic day 8.5; Itga5^tm1Hyn/Itga5^tm1Hyn (more

)
Note: Double labeled: red - Tfap2a; green - Casp3 (assay MGI:5789193).

Structure	Level	Pattern	Image	Note
TS13: outflow tract	Present		7K

7L ©

Specimen 7L: embryonic day 8.5; Itga5^tm1Hyn/Itga5^tm1Hyn (more

)
Note: Double labeled: red - Tfap2a; green - Casp3 (assay MGI:5789193).

Structure	Level	Pattern	Image	Note
TS13: neural crest	Present		7L
TS13: surface ectoderm	Present		7L

S1A ©

Specimen S1A: embryonic day 8.5 (more

)
Note: Double labeled: red - Tfap2a; green - Fn1 (assay MGI:5789190).

Structure	Level	Pattern	Image	Note
TS13: neural crest	Present	Regionally restricted	S1A	Expression in cardiac neural crest cells.

S1A1 ©

Specimen S1A1: embryonic day 8.5 (more

)
Note: Double labeled: red - Tfap2a; green - Fn1 (assay MGI:5789190).

Structure	Level	Pattern	Image	Note
TS13: neural crest	Present	Regionally restricted	S1A1	Expression in cardiac neural crest progenitors.

S1A2 ©

Specimen S1A2: embryonic day 8.5 (more

)
Note: Double labeled: red - Tfap2a; green - Fn1 (assay MGI:5789190).

Structure	Level	Pattern	Image	Note
TS13: migrating neural crest	Present	Regionally restricted	S1A2	Expression in mesenchymal cardiac neural crest cells.

S1B ©

Specimen S1B: embryonic day 8.5; Fn1^tm1Hyn/Fn1^tm1Hyn (more

)
Note: Double labeled: red - Tfap2a; green - Fn1 (assay MGI:5789190).

Structure	Level	Pattern	Image	Note
TS13: branchial arch mesenchyme	Present		S1B
TS13: surface ectoderm	Present		S1B

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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