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Gene Expression Data
Assay Details
Assay
Reference: J:198275 Watanabe N, et al., The REV7 subunit of DNA polymerase zeta is essential for primordial germ cell maintenance in the mouse. J Biol Chem. 2013 Apr 12;288(15):10459-71
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5789141
Gene symbol: H2ax
Gene name: H2A.X variant histone
Antibody: anti-phospho-H2A.X (Ser139)
Detection system: Secondary antibody coupled to horseradish peroxidase
Results
Specimen 7A Rev7+/+: embryonic day 9.5 (more )
Structure Level Pattern Image Note
TS15: hindgut Absent 7A pH2AX Rev7+/+
Specimen 7A Rev7-/-: embryonic day 9.5; Mad2l2tm1Ymu/Mad2l2tm1Ymu (more )
Structure Level Pattern Image Note
TS15: hindgut Present Regionally restricted 7A pH2AX Rev7-/- A number of immunopositive cells were noted. Compared to a serial section, some of the cells were also positive for Pou5f1.
Specimen 7B Rev7+/+: embryonic day 13.5 (more )
Structure Level Pattern Image Note
TS21: urogenital ridge Ambiguous 7B pH2AX Rev7+/+
Specimen 7B Rev7-/-: embryonic day 13.5; Mad2l2tm1Ymu/Mad2l2tm1Ymu (more )
Structure Level Pattern Image Note
TS21: urogenital ridge Present Regionally restricted 7B pH2AX Rev7-/- Expression was detected in many somatic cells around the genital ridge.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory