Probe:	Fgf8 probe71
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS17: forelimb bud apical ectodermal ridge	Present	Regionally restricted	4C	Expression in the AER in a similar pattern to that seen in wild type limbs. Lower expression in the anterior region of the AER.

Structure	Level	Pattern	Image	Note
TS17: forelimb bud apical ectodermal ridge	Present	Regionally restricted		Slight reduction in expression abserved in the posterior region of the AER in 2 of 10 forelimbs at E10.5

Structure	Level	Pattern	Image	Note
TS17: forelimb bud apical ectodermal ridge	Present	Widespread	4D	Expression in the entire AER.

Structure	Level	Pattern	Image	Note
TS19: handplate apical ectodermal ridge	Present	Regionally restricted	4G	Level of expression is lower and disturbed along the dorsoventral axis.

Structure	Level	Pattern	Image	Note
TS19: handplate apical ectodermal ridge	Present		4H

Structure	Level	Pattern	Image	Note
TS17: hindlimb bud apical ectodermal ridge	Present	Regionally restricted	4K	Expression in the AER in a similar pattern to that seen in wild type limbs. Reduced expression in the posterior part of the AER in all mutant hindlimbs.

Structure	Level	Pattern	Image	Note
TS17: hindlimb bud apical ectodermal ridge	Present	Widespread	4L	Expression in the entire AER.

Structure	Level	Pattern	Image	Note
TS19: handplate apical ectodermal ridge	Present	Regionally restricted	4P	Discontinuous expression in the AER.

Structure	Level	Pattern	Image	Note
TS19: footplate apical ectodermal ridge	Present		4Q