Nefm Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:153908 de Castro BM, et al., The vesicular acetylcholine transporter is required for neuromuscular development and function. Mol Cell Biol. 2009 Oct;29(19):5238-50
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:5788441
Gene symbol:	Nefm
Gene name:	neurofilament, medium polypeptide

Antibody:	Anti-Neurofilament 150
Detection system:	Secondary antibody coupled to Alexa Fluor 546

Results

Specimen 8 wt/wt: embryonic day 18.5 (more

)
Note: Double labeled: red - Nefm; green - alpha-bungarotoxin.

Structure	Level	Pattern	Image	Note
TS26: diaphragm	Present		8_wt/wt

Specimen 8 wt/del: embryonic day 18.5; Chat/Slc18a3^tm1.1Vpra/Chat⁺, Chat/Slc18a3^tm1.1Vpra/Slc18a3⁺ (more

)
Note: Double labeled: red - Nefm; green - alpha-bungarotoxin. This embryo was a single heterozygote. However, since this allele affects two genes, the MGI database schema requires that we need to enter an allele pair per affected gene (thus resulting in the animal looking like a double heterozygote).

Structure	Level	Pattern	Image	Note
TS26: diaphragm	Present		8_wt/del

Specimen 8 del/del: embryonic day 18.5; Chat/Slc18a3^tm1.1Vpra/Chat/Slc18a3^tm1.1Vpra, Chat/Slc18a3^tm1.1Vpra/Chat/Slc18a3^tm1.1Vpra (more

)
Note: Double labeled: red - Nefm; green - alpha-bungarotoxin. This embryo was a single homozygote. However, since this allele affects two genes, the MGI database schema requires that we need to enter an allele pair per affected gene (thus resulting in the animal looking like a double homozygote).

Structure	Level	Pattern	Image	Note
TS26: diaphragm	Present		8_del/del	Staining was detected. However, the del/del mice had an increase in axonal sprouting and branching.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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