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Gene Expression Data
Assay Details
Assay
Reference: J:153908 de Castro BM, et al., The vesicular acetylcholine transporter is required for neuromuscular development and function. Mol Cell Biol. 2009 Oct;29(19):5238-50
Assay type: RT-PCR
MGI Accession ID: MGI:5788331
Gene symbol: Chat
Gene name: choline O-acetyltransferase
Probe: Chat-pC, Chat-pD
Probe preparation: labelled with SYBR green
Assay notes: Quantitative RT-PCR. Data was normalized to beta-actin expression.
Results Image: 5C
 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified Amount Genetic BackgroundMutant Allele(s)Sex Note
wt/wt E18.5 TS26: brain Present Not Specified; total RNA Not Specified Not Specified
wt/del E18.5 TS26: brain Present (a) Not Specified; total RNA involves: 129S4/SvJae * C57BL/6J Chat/Slc18a3tm1.1Vpra/Chat+, Chat/Slc18a3tm1.1Vpra/Slc18a3+Not Specified This embryo was a single heterozygote. However, since this allele affects two genes, the MGI database schema requires that we need to enter an allele pair per affected gene (thus resulting in the animal looking like a double heterozygote).
del/del E18.5 TS26: brain Present (b) Not Specified; total RNA involves: 129S4/SvJae * C57BL/6J Chat/Slc18a3tm1.1Vpra/Chat/Slc18a3tm1.1Vpra, Chat/Slc18a3tm1.1Vpra/Chat/Slc18a3tm1.1VpraNot Specified This embryo was a single homozygote. However, since this allele affects two genes, the MGI database schema requires that we need to enter an allele pair per affected gene (thus resulting in the animal looking like a double homozygote).
Notes:
(a) Expression level was increased nearly two-fold in wt/del compared to control littermates.
(b) Expression level was increased nearly four-fold in del/del compared to control littermates.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
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