Gene Expression Data
Assay Details
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Assay
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Reference:
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J:84873
Ding YQ, et al., Formation of whisker-related principal sensory nucleus-based lemniscal pathway requires a paired homeodomain transcription factor, Drg11. J Neurosci. 2003 Aug 13;23(19):7246-54
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Assay type:
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In situ reporter (knock in)
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MGI Accession ID:
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MGI:5787777
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Gene symbol:
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Drgx
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Gene name:
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dorsal root ganglia homeobox
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Reporter:
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other (see notes)
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Antibody:
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anti-Neo
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Detection system:
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Secondary antibody coupled to Cy3
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Assay notes:
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Neomycin, included in the allele construct, was used as the reporter in this assay.
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Results
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Specimen
2A:
embryonic day 14.5; Drgxtm1And/Drgx +
(more  )
Note: (C) is a higher magnification of (A).
Specimen
2A:
(close  )
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Genetic Background:
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involves: 129S7/SvEvBrd
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Mutant Allele(s):
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Drgxtm1And/Drgx+
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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(C) is a higher magnification of (A).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: brainstem |
Present |
Regionally restricted |
2C
,
2A
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Expression is in the principal sensory nucleus.
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Specimen
2B:
embryonic day 14.5; Drgxtm1And/ Drgxtm1And
(more )
Note: (D) is a higher magnification of (B).
Specimen
2B:
(close )
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Genetic Background:
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involves: 129S7/SvEvBrd
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Mutant Allele(s):
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Drgxtm1And/Drgxtm1And
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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(D) is a higher magnification of (B).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: brainstem |
Present |
Regionally restricted |
2D
,
2B
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Expression in the principal sensory nucleus is reduced in the ventrolateral region.
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Specimen
2F:
embryonic day 16.5; Drgxtm1And/Drgx +
(more )
Specimen
2F:
(close )
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Genetic Background:
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involves: 129S7/SvEvBrd
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Mutant Allele(s):
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Drgxtm1And/Drgx+
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: trigeminal nucleus |
Present |
Regionally restricted |
2F
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Expression is in the principal sensory nucleus.
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Specimen
2G:
(close )
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Genetic Background:
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involves: 129S7/SvEvBrd
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Mutant Allele(s):
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Drgxtm1And/Drgxtm1And
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: trigeminal nucleus |
Present |
Regionally restricted |
2G
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Expression is in the principal sensory nucleus.
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Specimen
not shown E11.5 +/-:
embryonic day 11.5; Drgxtm1And/Drgx +
(more )
Specimen
not shown E11.5 +/-:
(close )
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Genetic Background:
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involves: 129S7/SvEvBrd
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Mutant Allele(s):
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Drgxtm1And/Drgx+
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: hindbrain |
Present |
Regionally restricted |
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Expression is in the principal sensory nucleus.
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Specimen
not shown E11.5 -/-:
(close )
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Genetic Background:
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involves: 129S7/SvEvBrd
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Mutant Allele(s):
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Drgxtm1And/Drgxtm1And
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: hindbrain |
Present |
Regionally restricted |
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Expression is in the principal sensory nucleus and is the same as in heterozygotes.
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Specimen
not shown E13.5 +/-:
embryonic day 13.5; Drgxtm1And/Drgx +
(more )
Specimen
not shown E13.5 +/-:
(close )
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Genetic Background:
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involves: 129S7/SvEvBrd
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Mutant Allele(s):
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Drgxtm1And/Drgx+
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: brainstem |
Present |
Regionally restricted |
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Expression is in the principal sensory nucleus.
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Specimen
not shown E13.5 -/-:
(close )
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Genetic Background:
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involves: 129S7/SvEvBrd
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Mutant Allele(s):
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Drgxtm1And/Drgxtm1And
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: brainstem |
Present |
Regionally restricted |
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Expression is in the principal sensory nucleus and is the same as in heterozygotes.
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Analysis Tools