Probe:	Foxp2 cDNA4
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS17: 1st branchial arch	Present	Regionally restricted	1C	Expression was excluded from the oral and anterior regions.

Structure	Level	Pattern	Image	Note
TS19: 1st branchial arch	Present	Regionally restricted	1G	Expressed in the mesenchyme, but not the epithelium. Partially overlapping with Foxp1, but smaller.
TS19: branchial arch mesenchyme	Present	Regionally restricted	1G	Expressed in the first pharyngeal arches.

Structure	Level	Pattern	Image	Note
TS19: 1st branchial arch	Present	Regionally restricted	1K	Expressed in the mesenchyme, but not the epithelium. Partially overlapping with Foxp1, but smaller.
TS19: branchial arch mesenchyme	Present	Regionally restricted	1K	Expressed in the first pharyngeal arches.

Structure	Level	Pattern	Image	Note
TS19: 1st branchial arch	Present	Regionally restricted	1O	Expressed in the mesenchyme, but not the epithelium. Partially overlapping with Foxp1, but smaller.
TS19: branchial arch mesenchyme	Present	Regionally restricted	1O	Expressed in the first pharyngeal arches.

Structure	Level	Pattern	Image	Note
TS20: face mesenchyme	Present	Regionally restricted	2B	Not expressed in the mesenchyme of the ventral (oral) domain of the palatal shelf.

Structure	Level	Pattern	Image	Note
TS20: head mesenchyme	Present	Regionally restricted	2G	Expression was strongest in the mesenchyme just under the eye.
TS20: face mesenchyme	Weak	Regionally restricted	2G	Expression extended ventrally into the area where Foxp1 was expressed.

Structure	Level	Pattern	Image	Note
TS20: head mesenchyme	Present	Regionally restricted	2L	In the posterior section, the expression appeared nearly complementary to that of Foxp1.

Structure	Level	Pattern	Image	Note
TS21: tooth mesenchyme	Absent		4B
TS21: palatal shelf mesenchyme	Ambiguous	Regionally restricted	4B	Not expressed in ventral (oral) domain of the palatal shelf.
TS21: face mesenchyme	Present		4B	Expressed mostly in the undifferentiated mesenchyme.

Structure	Level	Pattern	Image	Note
TS21: face mesenchyme	Present	Regionally restricted	4H	Reduced expression in the mesenchyme under the eye, with sharp boundaries. Mostly in the undifferentiated mesenchyme.

Structure	Level	Pattern	Image	Note
TS21: face mesenchyme	Present		4N	Expressed mostly in the undifferentiated mesenchyme.

Structure	Level	Pattern	Image	Note
TS21: face mesenchyme	Present		4T	Expressed mostly in the undifferentiated mesenchyme.

Structure	Level	Pattern	Image	Note
TS21: face mesenchyme	Present		4Y	Expressed mostly in the undifferentiated mesenchyme.

Structure	Level	Pattern	Image	Note
TS21: face mesenchyme	Present		4d	Expressed mostly in the undifferentiated mesenchyme.

Structure	Level	Pattern	Image	Note
TS21: face mesenchyme	Present		4i	Expressed mostly in the undifferentiated mesenchyme.

Structure	Level	Pattern	Image	Note
TS22: face mesenchyme	Present	Regionally restricted	6B	Further reduced expression. Expressed only in small areas just under the eye and at the root of the tongue.

Structure	Level	Pattern	Image	Note
TS22: face mesenchyme	Present	Regionally restricted	6G	Further reduced expression. Expressed only in small areas just under the eye and at the root of the tongue.

Structure	Level	Pattern	Image	Note
TS22: face mesenchyme	Present		6L

Structure	Level	Pattern	Image	Note
TS22: temporal bone petrous part	Present	Regionally restricted	6Q	Expressed in the developing temporo-mandibular joint.

Structure	Level	Pattern	Image	Note
TS23: jaw mesenchyme	Weak		7C
TS23: tooth mesenchyme	Absent		7C

Structure	Level	Pattern	Image	Note
TS23: jaw mesenchyme	Weak		7F

Structure	Level	Pattern	Image	Note
TS23: temporo-mandibular joint primordium	Weak	Regionally restricted	7I	Expressed in the articular disc.
TS23: jaw mesenchyme	Weak	Regionally restricted	7I	Expressed inthe mesenchyme around the condyle.

Structure	Level	Pattern	Image	Note
TS24: face mesenchyme	Absent