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Gene Expression Data
Assay Details
Assay
Reference: J:96963 Stemmler MP, et al., E-cadherin intron 2 contains cis-regulatory elements essential for gene expression. Development. 2005 Mar;132(5):965-76
Assay type: In situ reporter (knock in)
MGI Accession ID: MGI:5784500
Gene symbol: Cdh1
Gene name: cadherin 1
Reporter: lacZ
Direct Detection
Assay notes: Fixative was 1% formaldehyde/0.2% glutaraldehyde and a postfixation with 4% paraformaldehyde.
Results
Specimen 3A: embryonic day 6.5; Cdh1tm3.1Kem/Cdh1+ (more )
Structure Level Pattern Image Note
TS9: extraembryonic ectoderm Strong 3A
TS9: embryo ectoderm Present 3A

Specimen 3B: embryonic day 7.5; Cdh1tm3.1Kem/Cdh1+ (more )
Structure Level Pattern Image Note
TS11: embryo ectoderm Present 3B
TS11: embryo endoderm Present 3B
TS11: embryo mesoderm Absent 3B

Specimen 3C: embryonic day 8.5; Cdh1tm3.1Kem/Cdh1+ (more )
Structure Level Pattern Image Note
TS13: gut endoderm Very strong 3C
TS13: surface ectoderm Present 3C
TS13: yolk sac Present 3C

Specimen 3D: embryonic day 9.5; Cdh1tm3.1Kem/Cdh1+ (more )
Structure Level Pattern Image Note
TS15: gut endoderm Very strong 3D
TS15: surface ectoderm Present 3D
TS15: yolk sac Present 3D

Specimen 3E: embryonic day 10.5; Cdh1tm3.1Kem/Cdh1+ (more )
Structure Level Pattern Image Note
TS17: gut endoderm Very strong 3E
TS17: yolk sac Strong 3E
TS17: surface ectoderm Present 3E

Specimen 3F: embryonic day 7.5; Cdh1tm3.1Kem/Cdh1+ (more )
Structure Level Pattern Image Note
TS11: embryo endoderm Present 3F
TS11: embryo ectoderm Present 3F Expression levels appear higher in posterior ectoderm.
TS11: embryo mesoderm Absent 3F

Specimen 3G: embryonic day 10.5; Cdh1tm3.1Kem/Cdh1+ (more )
Structure Level Pattern Image Note
TS17: gut epithelium Strong 3G
TS17: pharynx Strong 3G
TS17: gut endoderm Present 3G

Specimen 3H: embryonic day 6.5; Cdh1tm3.2Kem/Cdh1+ (more )
Structure Level Pattern Image Note
TS9: embryo Absent 3H
TS9: extraembryonic ectoderm Absent 3H

Specimen 3I: embryonic day 7.5; Cdh1tm3.2Kem/Cdh1+ (more )
Structure Level Pattern Image Note
TS11: embryo Absent 3I
TS11: embryo ectoderm Absent 3I
TS11: embryo endoderm Absent 3I

Specimen 3J: embryonic day 8.5; Cdh1tm3.2Kem/Cdh1+ (more )
Structure Level Pattern Image Note
TS13: embryo Absent 3J
TS13: gut endoderm Absent 3J

Specimen 3K: embryonic day 9.5; Cdh1tm3.2Kem/Cdh1+ (more )
Structure Level Pattern Image Note
TS15: embryo Absent 3K
TS15: gut endoderm Absent 3K

Specimen 3L: embryonic day 10.5; Cdh1tm3.2Kem/Cdh1+ (more )
Structure Level Pattern Image Note
TS17: yolk sac Present 3L
TS17: forelimb bud apical ectodermal ridge Weak 3L
TS17: gut endoderm Absent 3L

Specimen 3M: embryonic day 7.5; Cdh1tm3.2Kem/Cdh1+ (more )
Structure Level Pattern Image Note
TS11: embryo Absent 3M
TS11: embryo ectoderm Absent 3M
TS11: embryo endoderm Absent 3M

Specimen 3N: embryonic day 10.5; Cdh1tm3.2Kem/Cdh1+ (more )
Structure Level Pattern Image Note
TS17: gut endoderm Absent 3N

Specimen not shown: embryonic day 11.0; Cdh1tm3.2Kem/Cdh1+ (more )
Structure Level Pattern Image Note
TS18: branchial arch Present Regionally restricted Expression is between the first and second branchial arch.

Specimen 4A: embryonic day 11.5; Cdh1tm3.1Kem/Cdh1+ (more )
Note: Overnight staining for B-gal expression.
Structure Level Pattern Image Note
TS19: surface ectoderm Strong 4A

Specimen 4B: embryonic day 12.5; Cdh1tm3.1Kem/Cdh1+ (more )
Note: Overnight staining for B-gal expression.
Structure Level Pattern Image Note
TS20: face skin Present 4B
TS20: skin Present 4B
TS20: vibrissa follicle Present 4B

Specimen 4C: embryonic day 16.5; Cdh1tm3.1Kem/Cdh1+ (more )
Note: 45 minute staining for B-gal expression.
Structure Level Pattern Image Note
TS24: skin Strong 4C

Specimen 4D: embryonic day 16.5; Cdh1tm3.1Kem/Cdh1+ (more )
Note: 45 minute staining for B-gal expression.
Structure Level Pattern Image Note
TS24: gut Strong 4D
TS24: pancreas Strong 4D
TS24: skin Strong 4D
TS24: stomach Strong 4D
TS24: thymus Strong 4D

Specimen 4E: embryonic day 16.5; Cdh1tm3.1Kem/Cdh1+ (more )
Note: Isolated organs. Overnight staining for B-gal expression.
Structure Level Pattern Image Note
TS24: gut Strong 4E
TS24: pancreas Strong 4E
TS24: stomach Strong 4E
TS24: thymus Strong 4E

Specimen 4F: embryonic day 11.5; Cdh1tm3.2Kem/Cdh1+ (more )
Note: Overnight staining for B-gal expression.
Structure Level Pattern Image Note
TS19: surface ectoderm Weak Regionally restricted 4F Expression is in the facial region.
TS19: branchial arch ectoderm Weak Regionally restricted 4F Expression is in the mandibular and maxillary components.
TS19: apical ectodermal ridge Weak 4F

Specimen 4G: embryonic day 12.5; Cdh1tm3.2Kem/Cdh1+ (more )
Note: Overnight staining for B-gal expression.
Structure Level Pattern Image Note
TS20: face skin Weak 4G
TS20: vibrissa follicle Weak 4G

Specimen 4H: embryonic day 16.5; Cdh1tm3.2Kem/Cdh1+ (more )
Note: 45 minute staining for B-gal expression.
Structure Level Pattern Image Note
TS24: skin Present 4H

Specimen 4I: embryonic day 16.5; Cdh1tm3.2Kem/Cdh1+ (more )
Note: 45 minute staining for B-gal expression.
Structure Level Pattern Image Note
TS24: skin Present 4I
TS24: esophagus Weak 4I
TS24: pancreas Weak 4I

Specimen 4J: embryonic day 16.5; Cdh1tm3.2Kem/Cdh1+ (more )
Note: Isolated organs. Overnight staining for B-gal expression.
Structure Level Pattern Image Note
TS24: lung epithelium Weak 4J
TS24: esophagus Present 4J
TS24: pancreas Present 4J

Specimen 4K: embryonic day 11.5; Cdh1tm3.1Kem/Cdh1+ (more )
Structure Level Pattern Image Note
TS19: lens vesicle Strong 4K
TS19: head surface ectoderm Strong Regionally restricted 4K Expression is in the ectoderm surrounding the eye.

Specimen 4L: embryonic day 11.5; Cdh1tm3.1Kem/Cdh1+ (more )
Structure Level Pattern Image Note
TS19: surface ectoderm Present 4L

Specimen 4M: embryonic day 11.5; Cdh1tm3.1Kem/Cdh1+ (more )
Structure Level Pattern Image Note
TS19: nasal pit Present 4M

Specimen 4N: embryonic day 11.5; Cdh1tm3.1Kem/Cdh1+ (more )
Structure Level Pattern Image Note
TS19: stomach Present 4N

Specimen 4O: embryonic day 11.5; Cdh1tm3.1Kem/Cdh1+ (more )
Structure Level Pattern Image Note
TS19: pancreas primordium Present 4O

Specimen 4P: embryonic day 11.5; Cdh1tm3.1Kem/Cdh1+ (more )
Structure Level Pattern Image Note
TS19: gut Present 4P

Specimen 4Q: embryonic day 11.5; Cdh1tm3.1Kem/Cdh1+ (more )
Structure Level Pattern Image Note
TS19: metanephros Present 4Q

Specimen 4R: embryonic day 11.5; Cdh1tm3.2Kem/Cdh1+ (more )
Structure Level Pattern Image Note
TS19: head surface ectoderm Absent 4R
TS19: lens vesicle Absent 4R

Specimen 4S: embryonic day 11.5; Cdh1tm3.2Kem/Cdh1+ (more )
Structure Level Pattern Image Note
TS19: surface ectoderm Trace Regionally restricted 4S Shown is an area where expression is completely absent (back skin). Very faint expression is seen in the facial region and at the apical ectodermal ridge.

Specimen 4T: embryonic day 11.5; Cdh1tm3.2Kem/Cdh1+ (more )
Structure Level Pattern Image Note
TS19: nasal pit Absent 4T

Specimen 4U: embryonic day 11.5; Cdh1tm3.2Kem/Cdh1+ (more )
Structure Level Pattern Image Note
TS19: stomach Absent 4U

Specimen 4V: embryonic day 11.5; Cdh1tm3.2Kem/Cdh1+ (more )
Structure Level Pattern Image Note
TS19: pancreas primordium Weak 4V

Specimen 4W: embryonic day 11.5; Cdh1tm3.2Kem/Cdh1+ (more )
Structure Level Pattern Image Note
TS19: gut Absent 4W

Specimen 4X: embryonic day 11.5; Cdh1tm3.2Kem/Cdh1+ (more )
Structure Level Pattern Image Note
TS19: metanephros Absent 4X

Specimen 5B +/flox: embryonic day 10.5; Cdh1tm3.1Kem/Cdh1+ (more )
Structure Level Pattern Image Note
TS17: yolk sac Strong 5B +/flox

Specimen 5B +/del: embryonic day 10.5; Cdh1tm3.2Kem/Cdh1+ (more )
Structure Level Pattern Image Note
TS17: yolk sac Strong 5B +/del

Specimen 5D left: embryonic day 12.5; Cdh1tm3.1Kem/Cdh1+ (more )
Structure Level Pattern Image Note
TS20: yolk sac Strong 5D +/flox

Specimen 5D right: embryonic day 12.5; Cdh1tm3.2Kem/Cdh1+ (more )
Structure Level Pattern Image Note
TS20: yolk sac Strong 5D +/del

Specimen 6A: embryonic day 10.5; Cdh1tm3.1Kem/Cdh1+ (more )
Structure Level Pattern Image Note
TS17: lens vesicle Very strong 6A

Specimen 6B: embryonic day 10.5; Cdh1tm3.2Kem/Cdh1+ (more )
Structure Level Pattern Image Note
TS17: lens vesicle Absent 6B

Specimen 6C: embryonic day 12.5; Cdh1tm3.1Kem/Cdh1+ (more )
Structure Level Pattern Image Note
TS20: lens vesicle Very strong 6C

Specimen 6D: embryonic day 12.5; Cdh1tm3.2Kem/Cdh1+ (more )
Structure Level Pattern Image Note
TS20: lens vesicle Absent 6D

Specimen 6E: embryonic day 14.5; Cdh1tm3.1Kem/Cdh1+ (more )
Structure Level Pattern Image Note
TS22: lens Very strong 6E

Specimen 6F: embryonic day 14.5; Cdh1tm3.2Kem/Cdh1+ (more )
Structure Level Pattern Image Note
TS22: lens Absent 6F

Specimen 6G: embryonic day 16.5; Cdh1tm3.1Kem/Cdh1+ (more )
Structure Level Pattern Image Note
TS24: salivary gland Strong 6G

Specimen 6H: embryonic day 16.5; Cdh1tm3.2Kem/Cdh1+ (more )
Structure Level Pattern Image Note
TS24: salivary gland Absent 6H

Specimen 6I: embryonic day 16.5; Cdh1tm3.1Kem/Cdh1+ (more )
Structure Level Pattern Image Note
TS24: salivary gland Strong 6I

Specimen 6J: embryonic day 16.5; Cdh1tm3.1Kem/Cdh1+ (more )
Structure Level Pattern Image Note
TS24: thyroid gland Present 6J

Specimen 6K: embryonic day 16.5; Cdh1tm3.1Kem/Cdh1+ (more )
Structure Level Pattern Image Note
TS24: trachea Present 6K

Specimen 6L: embryonic day 16.5; Cdh1tm3.1Kem/Cdh1+ (more )
Structure Level Pattern Image Note
TS24: meninges Present 6L

Specimen 6M: embryonic day 16.5; Cdh1tm3.2Kem/Cdh1+ (more )
Structure Level Pattern Image Note
TS24: salivary gland Absent 6M

Specimen 6N: embryonic day 16.5; Cdh1tm3.2Kem/Cdh1+ (more )
Structure Level Pattern Image Note
TS24: thyroid gland Weak 6N

Specimen 6O: embryonic day 16.5; Cdh1tm3.2Kem/Cdh1+ (more )
Structure Level Pattern Image Note
TS24: trachea Weak 6O

Specimen 6P: embryonic day 16.5; Cdh1tm3.2Kem/Cdh1+ (more )
Structure Level Pattern Image Note
TS24: meninges Weak 6P

Specimen S2 I: embryonic day 5.5; Cdh1tm6Kem/Cdh1tm6Kem (more )
Structure Level Pattern Image Note
TS8: conceptus Present S2I

Specimen S2 J: embryonic day 6.5; Cdh1tm6Kem/Cdh1tm6Kem (more )
Structure Level Pattern Image Note
TS9: extraembryonic ectoderm Present S2J

Specimen S2 K: embryonic day 7.5; Cdh1tm6Kem/Cdh1tm6Kem (more )
Structure Level Pattern Image Note
TS11: embryo ectoderm Present S2K

Specimen S2 L: embryonic day 8.5; Cdh1tm6Kem/Cdh1tm6Kem (more )
Structure Level Pattern Image Note
TS13: gut endoderm Present S2L

Specimen S2 M: embryonic day 9.5; Cdh1tm6Kem/Cdh1tm6Kem (more )
Structure Level Pattern Image Note
TS15: gut endoderm Present S2M
TS15: surface ectoderm Present S2M

Specimen S2 N: embryonic day 10.5; Cdh1tm6Kem/Cdh1tm6Kem (more )
Structure Level Pattern Image Note
TS17: gut endoderm Present S2N
TS17: lens vesicle Present S2N
TS17: surface ectoderm Present S2N

Specimen S2 O: embryonic day 11.5; Cdh1tm6Kem/Cdh1tm6Kem (more )
Note: Inset shows a higher magnification of the ventral midbrain.
Structure Level Pattern Image Note
TS19: midbrain Present S2O , S2O inset Expression is in the ventral midbrain.

Specimen S2 P: embryonic day 12.5; Cdh1tm6Kem/Cdh1tm6Kem (more )
Structure Level Pattern Image Note
TS20: skin Present S2P

Specimen S2 Q: embryonic day 14.5; Cdh1tm6Kem/Cdh1tm6Kem (more )
Structure Level Pattern Image Note
TS22: hair follicle Present S2Q

Specimen S2 R: embryonic day 16.5; Cdh1tm6Kem/Cdh1tm6Kem (more )
Structure Level Pattern Image Note
TS24: skin Present S2R

Specimen S2 S: embryonic day 14.5; Cdh1tm6Kem/Cdh1tm6Kem (more )
Structure Level Pattern Image Note
TS22: gut epithelium Present S2S

Specimen S2 T: embryonic day 14.5; Cdh1tm6Kem/Cdh1tm6Kem (more )
Structure Level Pattern Image Note
TS22: gut epithelium Present S2T

Specimen S2 U: embryonic day 7.5; Cdh1tm6Kem/Cdh1tm6Kem (more )
Structure Level Pattern Image Note
TS11: embryo ectoderm Present S2U

Specimen S2 V: embryonic day 7.5; Cdh1tm6Kem/Cdh1tm6Kem (more )
Structure Level Pattern Image Note
TS11: embryo ectoderm Present S2V

Specimen S2 W: embryonic day 8.0; Cdh1tm6Kem/Cdh1tm6Kem (more )
Structure Level Pattern Image Note
TS12: embryo Present S2W

Specimen S2 X: embryonic day 8.0; Cdh1tm6Kem/Cdh1tm6Kem (more )
Structure Level Pattern Image Note
TS12: embryo Present S2X

Specimen S2 Y: embryonic day 11.5; Cdh1tm6Kem/Cdh1tm6Kem (more )
Structure Level Pattern Image Note
TS19: midbrain Present Regionally restricted S2Y Expression is in the ventral midbrain.


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last database update
05/07/2024
MGI 6.23
The Jackson Laboratory