Gene Expression Data
Assay Details
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Assay
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Reference:
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J:138347
Wang J, et al., Expression pattern of serine protease inhibitor kazal type 3 (Spink3) during mouse embryonic development. Histochem Cell Biol. 2008 Aug;130(2):387-97
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Assay type:
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In situ reporter (knock in)
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MGI Accession ID:
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MGI:5780834
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Gene symbol:
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Spink1
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Gene name:
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serine peptidase inhibitor, Kazal type 1
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Reporter:
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lacZ
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Direct Detection
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Assay notes:
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Specimens were fixed in 1% formaldehyde, 0.2% glutaraldehyde.
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Results
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Specimen
2B:
(close  )
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Genetic Background:
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involves: C57BL/6 * C57BL/6J * CBA
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Mutant Allele(s):
|
Spink1tm2Kymm/Spink1+
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Age:
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embryonic day 9.5
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Sex:
|
Not Specified
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Type:
|
whole mount
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Fixation:
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Other - See Notes
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Specimen
2C:
(close )
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Genetic Background:
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involves: C57BL/6 * C57BL/6J * CBA
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Mutant Allele(s):
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Spink1tm2Kymm/Spink1+
|
|
Age:
|
embryonic day 9.5
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Sex:
|
Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: gut epithelium |
Weak |
Regionally restricted |
2C
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Relatively sparce expression in cells alng the cavity lining, spreading bi-directionally to the foregut and the midgut.
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| TS15: foregut-midgut junction |
Present |
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2C
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Considerable expression in the foregut/midgut junction and succeeding midgut.
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| TS15: midgut |
Present |
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2C
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Considerable expression in the foregut/midgut junction and succeeding midgut.
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Specimen
2D:
(close )
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Genetic Background:
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involves: C57BL/6 * C57BL/6J * CBA
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Mutant Allele(s):
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Spink1tm2Kymm/Spink1+
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|
Age:
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embryonic day 9.5
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|
Sex:
|
Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Specimen
2E:
(close )
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Genetic Background:
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involves: C57BL/6 * C57BL/6J * CBA
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Mutant Allele(s):
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Spink1tm2Kymm/Spink1+
|
|
Age:
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embryonic day 9.5
|
|
Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: future brain |
Present |
Diffuse |
2E
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Expression in cells within the neuroepithelium of the forebrain-midbrain junction.
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Specimen
2F:
(close )
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Genetic Background:
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involves: C57BL/6 * C57BL/6J * CBA
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Mutant Allele(s):
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Spink1tm2Kymm/Spink1+
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Specimen
3C:
(close )
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Genetic Background:
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involves: C57BL/6 * C57BL/6J * CBA
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Mutant Allele(s):
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Spink1tm2Kymm/Spink1+
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Specimen
3G:
(close )
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Genetic Background:
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involves: C57BL/6 * C57BL/6J * CBA
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Mutant Allele(s):
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Spink1tm2Kymm/Spink1+
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|
Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Specimen
3K:
(close )
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Genetic Background:
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involves: C57BL/6 * C57BL/6J * CBA
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Mutant Allele(s):
|
Spink1tm2Kymm/Spink1+
|
|
Age:
|
embryonic day 13.5
|
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: pancreas |
Present |
Regionally restricted |
3K
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Expression within a relatively limited area of the pancreas.
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Specimen
3O:
(close )
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Genetic Background:
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involves: C57BL/6 * C57BL/6J * CBA
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Mutant Allele(s):
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Spink1tm2Kymm/Spink1+
|
|
Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: pancreatic acinus |
Present |
Regionally restricted |
3O
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Prominent expression. Authors report restrictive expression in dispersed acinar cells surrounding the lumen of the acinus.
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| TS23: endocrine pancreas |
Absent |
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3O
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Specimen
3S:
(close )
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Genetic Background:
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involves: C57BL/6 * C57BL/6J * CBA
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Mutant Allele(s):
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Spink1tm2Kymm/Spink1+
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Age:
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embryonic day 17.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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| Structure |
Level |
Pattern |
Image |
Note |
| TS25: pancreatic acinus |
Present |
Regionally restricted |
3S
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Prominent expression. Authors report restrictive expression in dispersed acinar cells surrounding the lumen of the acinus.
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| TS25: endocrine pancreas |
Absent |
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3S
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Specimen
4A:
(close )
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Genetic Background:
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involves: C57BL/6 * C57BL/6J * CBA
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Mutant Allele(s):
|
Spink1tm2Kymm/Spink1+
|
|
Age:
|
embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Other - See Notes
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: intestine |
Present |
Regionally restricted |
4A
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Expression only in large intestine, most likely including the sigmoid colon and rectum.
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Specimen
4B:
(close )
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Genetic Background:
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involves: C57BL/6 * C57BL/6J * CBA
|
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Mutant Allele(s):
|
Spink1tm2Kymm/Spink1+
|
|
Age:
|
embryonic day 11.5
|
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Other - See Notes
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS19: intestine |
Present |
Regionally restricted |
4B
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Expression only in large intestine, most likely including the sigmoid colon and rectum.
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Specimen
4D:
(close )
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Genetic Background:
|
involves: C57BL/6 * C57BL/6J * CBA
|
|
Mutant Allele(s):
|
Spink1tm2Kymm/Spink1+
|
|
Age:
|
embryonic day 12.5
|
|
Sex:
|
Not Specified
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|
Type:
|
whole mount
|
|
Fixation:
|
Other - See Notes
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS20: intestine |
Present |
Regionally restricted |
4D
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Expression only in large intestine, most likely including the sigmoid colon and rectum.
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Specimen
4E:
(close )
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Genetic Background:
|
involves: C57BL/6 * C57BL/6J * CBA
|
|
Mutant Allele(s):
|
Spink1tm2Kymm/Spink1+
|
|
Age:
|
embryonic day 12.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
Other - See Notes
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS20: intestine |
Present |
Regionally restricted |
4E
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Expression only in large intestine, most likely including the sigmoid colon and rectum.
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Specimen
5A:
(close )
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Genetic Background:
|
involves: C57BL/6 * C57BL/6J * CBA
|
|
Mutant Allele(s):
|
Spink1tm2Kymm/Spink1+
|
|
Age:
|
embryonic day 11.5
|
|
Sex:
|
Not Specified
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|
Type:
|
whole mount
|
|
Fixation:
|
Other - See Notes
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Specimen
5B:
(close )
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Genetic Background:
|
involves: C57BL/6 * C57BL/6J * CBA
|
|
Mutant Allele(s):
|
Spink1tm2Kymm/Spink1+
|
|
Age:
|
embryonic day 11.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Other - See Notes
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Specimen
5E:
(close )
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Genetic Background:
|
involves: C57BL/6 * C57BL/6J * CBA
|
|
Mutant Allele(s):
|
Spink1tm2Kymm/Spink1+
|
|
Age:
|
embryonic day 12.5
|
|
Sex:
|
Not Specified
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Type:
|
whole mount
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Fixation:
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Other - See Notes
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Specimen
5F:
(close )
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Genetic Background:
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involves: C57BL/6 * C57BL/6J * CBA
|
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Mutant Allele(s):
|
Spink1tm2Kymm/Spink1+
|
|
Age:
|
embryonic day 12.5
|
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Sex:
|
Not Specified
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Type:
|
section
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Fixation:
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Other - See Notes
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Specimen
5I:
(close )
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Genetic Background:
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involves: C57BL/6 * C57BL/6J * CBA
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Mutant Allele(s):
|
Spink1tm2Kymm/Spink1+
|
|
Age:
|
embryonic day 13.5
|
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Sex:
|
Not Specified
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Type:
|
whole mount
|
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Fixation:
|
Other - See Notes
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Specimen
5J:
(close )
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Genetic Background:
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involves: C57BL/6 * C57BL/6J * CBA
|
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Mutant Allele(s):
|
Spink1tm2Kymm/Spink1+
|
|
Age:
|
embryonic day 13.5
|
|
Sex:
|
Not Specified
|
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Type:
|
section
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Fixation:
|
Other - See Notes
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Specimen
5M:
(close )
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Genetic Background:
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involves: C57BL/6 * C57BL/6J * CBA
|
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Mutant Allele(s):
|
Spink1tm2Kymm/Spink1+
|
|
Age:
|
embryonic day 15.5
|
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Sex:
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Male
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Type:
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whole mount
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Fixation:
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Other - See Notes
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: mesonephros |
Present |
Regionally restricted |
5M
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Authors state expression was observed in the mesonephros or the cranial region of the male urogenital ridge.
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Specimen
5N:
(close )
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Genetic Background:
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involves: C57BL/6 * C57BL/6J * CBA
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Mutant Allele(s):
|
Spink1tm2Kymm/Spink1+
|
|
Age:
|
embryonic day 15.5
|
|
Sex:
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Male
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Type:
|
section
|
|
Fixation:
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Other - See Notes
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: mesonephros |
Present |
Regionally restricted |
5N
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Authors state expression was observed in the mesonephros or the cranial region of the male urogenital ridge.
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Specimen
5Q:
(close )
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Genetic Background:
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involves: C57BL/6 * C57BL/6J * CBA
|
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Mutant Allele(s):
|
Spink1tm2Kymm/Spink1+
|
|
Age:
|
embryonic day 17.5
|
|
Sex:
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Male
|
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Type:
|
whole mount
|
|
Fixation:
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Other - See Notes
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| Structure |
Level |
Pattern |
Image |
Note |
| TS25: epididymis |
Present |
Regionally restricted |
5Q
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Expression clearly observed in the differentiated epididymis. Expression in the epithelium of the ductus epididymis.
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Specimen
5R:
(close )
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Genetic Background:
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involves: C57BL/6 * C57BL/6J * CBA
|
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Mutant Allele(s):
|
Spink1tm2Kymm/Spink1+
|
|
Age:
|
embryonic day 17.5
|
|
Sex:
|
Male
|
|
Type:
|
section
|
|
Fixation:
|
Other - See Notes
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS25: epididymis |
Present |
Regionally restricted |
5R
|
Expression clearly observed in the differentiated epididymis. Expression in the epithelium of the ductus epididymis.
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Specimen
E9.5 not shown:
embryonic day 9.5; Spink1tm2Kymm/Spink1 +
(more  )
Specimen
E9.5 not shown:
(close )
|
Genetic Background:
|
involves: C57BL/6 * C57BL/6J * CBA
|
|
Mutant Allele(s):
|
Spink1tm2Kymm/Spink1+
|
|
Age:
|
embryonic day 9.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
Not Specified
|
|
Fixation:
|
Other - See Notes
|
Specimen
E11.5 not shown:
embryonic day 11.5; Spink1tm2Kymm/Spink1 +
(more )
Specimen
E11.5 not shown:
(close )
|
Genetic Background:
|
involves: C57BL/6 * C57BL/6J * CBA
|
|
Mutant Allele(s):
|
Spink1tm2Kymm/Spink1+
|
|
Age:
|
embryonic day 11.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
Not Specified
|
|
Fixation:
|
Other - See Notes
|
Specimen
E12.5 not shown:
embryonic day 12.5; Spink1tm2Kymm/Spink1 +
(more )
Specimen
E12.5 not shown:
(close )
|
Genetic Background:
|
involves: C57BL/6 * C57BL/6J * CBA
|
|
Mutant Allele(s):
|
Spink1tm2Kymm/Spink1+
|
|
Age:
|
embryonic day 12.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
Not Specified
|
|
Fixation:
|
Other - See Notes
|
Specimen
E13.5 not shown:
embryonic day 13.5; Spink1tm2Kymm/Spink1 +
(more )
Specimen
E13.5 not shown:
(close )
|
Genetic Background:
|
involves: C57BL/6 * C57BL/6J * CBA
|
|
Mutant Allele(s):
|
Spink1tm2Kymm/Spink1+
|
|
Age:
|
embryonic day 13.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
Not Specified
|
|
Fixation:
|
Other - See Notes
|
Specimen
E15.5 not shown:
embryonic day 15.5; Spink1tm2Kymm/Spink1 +
(more )
Specimen
E15.5 not shown:
(close )
|
Genetic Background:
|
involves: C57BL/6 * C57BL/6J * CBA
|
|
Mutant Allele(s):
|
Spink1tm2Kymm/Spink1+
|
|
Age:
|
embryonic day 15.5
|
|
Sex:
|
Female
|
|
Type:
|
Not Specified
|
|
Fixation:
|
Other - See Notes
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS23: mesonephros |
Present |
Regionally restricted |
|
Authors report expression in the cranial portionof the ridge in female.
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| TS23: genital tubercle |
Present |
|
|
Moderate to high expression in the surface layer of the genital swelling between 12.5 and 15.5 dpc.
|
Specimen
E17.5 female not shown:
embryonic day 17.5; Spink1tm2Kymm/Spink1 +
(more )
Specimen
E17.5 female not shown:
(close )
|
Genetic Background:
|
involves: C57BL/6 * C57BL/6J * CBA
|
|
Mutant Allele(s):
|
Spink1tm2Kymm/Spink1+
|
|
Age:
|
embryonic day 17.5
|
|
Sex:
|
Female
|
|
Type:
|
Not Specified
|
|
Fixation:
|
Other - See Notes
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS25: paramesonephric duct |
Ambiguous |
|
|
Authors state expression was hardly detected in the Mullerian duct of the female.
|
Specimen
E17.5 male not shown:
embryonic day 17.5; Spink1tm2Kymm/Spink1 +
(more )
Specimen
E17.5 male not shown:
(close )
|
Genetic Background:
|
involves: C57BL/6 * C57BL/6J * CBA
|
|
Mutant Allele(s):
|
Spink1tm2Kymm/Spink1+
|
|
Age:
|
embryonic day 17.5
|
|
Sex:
|
Male
|
|
Type:
|
Not Specified
|
|
Fixation:
|
Other - See Notes
|
Specimen
8 weeks not shown:
postnatal week 8; Spink1tm2Kymm/Spink1 +
(more )
Specimen
8 weeks not shown:
(close )
|
Genetic Background:
|
involves: C57BL/6 * C57BL/6J * CBA
|
|
Mutant Allele(s):
|
Spink1tm2Kymm/Spink1+
|
|
Age:
|
postnatal week 8
|
|
Sex:
|
Male
|
|
Type:
|
Not Specified
|
|
Fixation:
|
Other - See Notes
|
|
Analysis Tools
