Tg(Scgb1a1-CFTR)6Unc
Transgene Detail
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| Symbol: |
Tg(Scgb1a1-CFTR)6Unc |
| Name: |
transgene insertion 6, University of North Carolina |
| MGI ID: |
MGI:3606222 |
| Transgene: |
Tg(Scgb1a1-CFTR)6Unc Location: unknown
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| Alliance: |
Tg(Scgb1a1-CFTR)6Unc page
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| Transgene Type: |
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Transgenic (Inserted expressed sequence) |
| Mutation: |
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Insertion
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Mutation details: Approximately 2330 base pairs of 5' flanking sequence from the rat airway-specific Scgb1a1 (also called the Clara cell secretory protein) gene were inserted into the pTG1 vector upstream of exon 1 (104 base pairs, including the transcription initiation site), intron 1 (947 base pairs) and 10 base pairs of exon 2 from the mouse transthyretin gene; the protein-coding region of the normal human CFTR cDNA, inserted just downstream of the transthyretin exon 2 splice acceptor, is followed by the SV40 early-region polyadenylation signal. Expression is demonstrated by an elevated basal current that is sensitive to CFTR inhibitors; however, its lack of forskolin dependence suggests constitutive activity.
(J:183328)
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| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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| Original: |
J:183328 Grubb BR, et al., Transgenic hCFTR expression fails to correct beta-ENaC mouse lung disease. Am J Physiol Lung Cell Mol Physiol. 2012 Jan;302(2):L238-47 |
| All: |
1 reference(s) |
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Analysis Tools
