Probe:	Cyr61 cDNA1
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS13: trophoblast giant cell	Present		3C
TS28: decidua	Strong	Regionally restricted	3C	Expressed in the decidual cells located above the cone.
TS13: ectoplacental cone	Strong	Regionally restricted	3C	Expressed in the region containing the ectoplacental glycogen cells that will differentiate into trophosphongium.

Structure	Level	Pattern	Image	Note
TS20: spongiotrophoblast	Strong	Regionally restricted	3E	Expressed in the central layer.
TS28: decidua	Strong	Regionally restricted	3E	Expressed in the compact decidua overlaying the spongiotrophoblasts.

Structure	Level	Pattern	Image	Note
TS19: spongiotrophoblast	Strong	Regionally restricted	3F
TS19: trophoblast giant cell	Strong	Regionally restricted	3F
TS28: decidua	Present	Regionally restricted	3F	Expressed in the compact decidua overlaying the spongiotrophoblasts.
TS19: labyrinthine zone	Absent		3F

Structure	Level	Pattern	Image	Note
TS20: sclerotome	Present	Widespread	4A/B	Expressed in the regions of sclerotome along the developing vertebral column.
TS20: dorsal aorta	Present		4A/B
TS20: pulmonary trunk	Present		4A/B
TS20: ascending aorta	Present		4A/B	The bulbus arteriosus.

Structure	Level	Pattern	Image	Note
TS21: sclerotome	Present	Widespread	4C/D	Expressed along the developing vertebral column.
TS21: sphenoid bone pre-cartilage condensation	Present		4C/D	Expressed in the aboral and oral sphenoid cartilages.
TS21: ascending aorta	Present		4C/D	Expressed in the smooth muscle walls.
TS21: pulmonary trunk	Present		4C/D	Expressed in the smooth muscle walls.
TS21: umbilical artery	Present		4C/D	Expressed in the smooth muscle walls.

Structure	Level	Pattern	Image	Note
TS22: sclerotome	Present	Regionally restricted	4E/F	Expression was most prominent in the posterior of the embryo in regions where new vertebral formation is occurring.

Structure	Level	Pattern	Image	Note
TS21: pelvic girdle pre-cartilage condensation	Present		5A/B
TS21: rib cartilage condensation	Present		5A/B
TS21: cervical vertebral cartilage condensation	Present		5A/B	Lower levels.
TS21: Meckel's cartilage	Present		5A/B

Structure	Level	Pattern	Image	Note
TS21: rib cartilage condensation	Present		5C/D

Structure	Level	Pattern	Image	Note
TS21: Meckel's cartilage	Present		5E/F

Structure	Level	Pattern	Image	Note
TS22: metatarsus cartilage condensation	Present		5G/H
TS22: phalanx cartilage condensation	Present		5G/H

Structure	Level	Pattern	Image	Note
TS23: ethmoid bone primordium	Present		6B/C

Structure	Level	Pattern	Image	Note
TS23: clavicle	Present	Regionally restricted	6D/E	Expression is more prominent in the core chondrocytes than in the surrounding periosteum.

Structure	Level	Pattern	Image	Note
TS21: rib cartilage condensation	Present	Regionally restricted	6F/G	More prominent expression in central loosely packed chondrocytes rather than in the perichondrium.

Structure	Level	Pattern	Image	Note
TS22: ascending aorta	Present	Regionally restricted	7A/B	Expressed in the smooth muscle walls.
TS22: pulmonary trunk	Present	Regionally restricted	7A/B	Expressed in the smooth muscle walls.
TS22: sternum	Present	Regionally restricted	7A/B	Expressed in the cartilages.

Structure	Level	Pattern	Image	Note
TS21: sclerotome	Present	Widespread	7C/D	Expressed in the regions of sclerotome along the developing vertebral column.
TS21: dorsal aorta	Present		7C/D
TS21: umbilical artery	Present		7C/D

Structure	Level	Pattern	Image	Note
TS21: basioccipital pre-cartilage condensation	Present
TS21: forelimb cartilage condensation	Present
TS21: hindlimb cartilage condensation	Present
TS21: hindlimb digit pre-cartilage condensation	Present

Structure	Level	Pattern	Image	Note
TS23: forelimb long bone	Present			Expressed in the developing cartilages.
TS23: hindlimb long bone	Present			Expressed in the developing cartilages.
TS23: pectoral girdle skeleton	Present			Expressed in the developing cartilages.
TS23: pelvic girdle cartilage condensation	Present			Expressed in the developing cartilages.