Tg(tetO/Prnp-GLRA1*,-EYFP)9511Jjan
Transgene Detail
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Symbol: |
Tg(tetO/Prnp-GLRA1*,-EYFP)9511Jjan |
Name: |
transgene insertion 9511, Joanna L Jankowsky |
MGI ID: |
MGI:5779809 |
Synonyms: |
TRE-GlyCl 9511 |
Transgene: |
Tg(tetO/Prnp-GLRA1*,-EYFP)9511Jjan Location: unknown
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Alliance: |
Tg(tetO/Prnp-GLRA1*,-EYFP)9511Jjan page
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Strain of Origin: |
(C57BL/6J x C3H/HeJ)F2
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Transgene Type: |
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Transgenic (Humanized sequence, Inducible, Reporter) |
Inducer: |
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doxycycline/tetracycline |
Mutation: |
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Insertion
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Mutation details: The transgene has a Tet-responsive element 3G promoter and the mouse prion protein non-coding exons 1-2 upstream of a cassette containing an enhanced yellow fluorescent reporter (EYFP) flanked on either side by a human glycine receptor alpha1 (GLRA1) cDNA sequence harboring the point mutations F207A (to eliminate endogenous ligand [glycine] sensitivity) and A288G (to increase ivermectin sensitivity almost 100-fold), followed by the mouse prion protein non-coding exon 3 and the woodchuck hepatitis post-transcriptional regulatory element (WPRE). To achieve tricistronic expression of the point mutations and EYFP, they are separated by T2A peptide sequences (from Thosea asigna virus). Lines 9487 and 9511 display the weakest overall signal.
(J:232624)
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Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
Carrying this Mutation: |
Mouse Strains: 1 strain available
Cell Lines: 0 lines available
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Original: |
J:232624 Zhao R, et al., Impaired Recall of Positional Memory following Chemogenetic Disruption of Place Field Stability. Cell Rep. 2016 Jul 19;16(3):793-804 |
All: |
1 reference(s) |
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