Probe:	Fam181b cDNA
Probe preparation:	Antisense labelled with digoxigenin RNA
Assay notes:	Specimens were postfixed in 4% paraformaldehyde.

Structure	Level	Pattern	Image	Note
TS11: future brain neural fold	Present		3A

Structure	Level	Pattern	Image	Note
TS11: future brain neural fold	Present		3A'

Structure	Level	Pattern	Image	Note
TS11: future brain neural fold	Present		3B

Structure	Level	Pattern	Image	Note
TS11: future brain neural fold	Present		3B'

Structure	Level	Pattern	Image	Note
TS12: future hindbrain	Present		3C
TS12: future midbrain	Present		3C
TS12: trunk unsegmented mesenchyme	Present		3C

Structure	Level	Pattern	Image	Note
TS12: future hindbrain	Present		3C'
TS12: future midbrain	Present		3C'
TS12: trunk unsegmented mesenchyme	Present		3C'

Structure	Level	Pattern	Image	Note
TS14: future hindbrain roof plate	Present		3D
TS14: future midbrain	Present		3D
TS14: future telencephalon	Present		3D
TS14: trunk unsegmented mesenchyme	Present		3D
TS14: neural tube	Present	Regionally restricted	3D	Expression is in the medial portion extending from the caudal end to the otic vesicle.
TS14: mesenchyme derived from lateral plate	Present	Non-Uniform	3D	Expression is strongest at the level of the prospective forelimb bud.
TS14: future hindbrain lateral wall	Absent		3D

Structure	Level	Pattern	Image	Note
TS14: future hindbrain roof plate	Present		3D'
TS14: future midbrain	Present		3D'
TS14: future telencephalon	Present		3D'
TS14: trunk unsegmented mesenchyme	Present		3D'
TS14: neural tube	Present	Regionally restricted	3D'	Expression is in the medial portion extending from the caudal end to the otic vesicle, there is a gap of expression at the level of the otic vesicle.
TS14: mesenchyme derived from lateral plate	Present	Non-Uniform	3D'	Expression is strongest at the level of the prospective forelimb bud.
TS14: future hindbrain lateral wall	Absent		3D'

Structure	Level	Pattern	Image	Note
TS14: future hindbrain roof plate	Present		3D''
TS14: future midbrain	Present		3D''
TS14: trunk unsegmented mesenchyme	Present		3D''
TS14: future hindbrain lateral wall	Absent		3D''

Structure	Level	Pattern	Image	Note
TS15: future midbrain	Present		3E
TS15: neural tube roof plate	Present		3E
TS15: future hindbrain roof plate	Present	Regionally restricted	3E	There is a gap of expression at the level of the otic vesicle.
TS15: forelimb bud	Present	Regionally restricted	3E	Expression is in a domain in the medial portion of the limb bud.
TS15: mesenchyme derived from lateral plate	Present	Regionally restricted	3E	Expression is at the level of the prospective hindlimb bud.
TS15: 1st branchial arch	Present	Regionally restricted	3E	Expression is in a small domain in the anterior portion.

Structure	Level	Pattern	Image	Note
TS15: future hindbrain roof plate	Present		3E'
TS15: future midbrain	Present		3E'
TS15: neural tube roof plate	Present		3E'

Structure	Level	Pattern	Image	Note
TS15: future hindbrain roof plate	Present		3E''
TS15: future midbrain	Present		3E''
TS15: neural tube roof plate	Present		3E''

Structure	Level	Pattern	Image	Note
TS16: dermomyotome	Present		3F
TS16: dorsal root ganglion	Present		3F
TS16: future hindbrain roof plate	Present		3F
TS16: future midbrain	Present		3F
TS16: neural tube roof plate	Present		3F
TS16: forelimb bud	Present	Regionally restricted	3F	Expression is in a medial and in a more distal domain of the limb bud.
TS16: hindlimb bud	Present	Regionally restricted	3F	Expression is in a domain in the medial portion of the limb bud.

Structure	Level	Pattern	Image	Note
TS16: dermomyotome	Present		3F'
TS16: dorsal root ganglion	Present		3F'
TS16: future hindbrain roof plate	Present		3F'
TS16: future midbrain	Present		3F'
TS16: neural tube roof plate	Present		3F'
TS16: forelimb bud	Present	Regionally restricted	3F'	Expression is in a medial and in a more distal domain of the limb bud.
TS16: hindlimb bud	Present	Regionally restricted	3F'	Expression is in a domain in the medial portion of the limb bud.

Structure	Level	Pattern	Image	Note
TS18: dermomyotome	Present		3G
TS18: hindbrain roof plate	Present		3G
TS18: midbrain	Present		3G
TS18: neural tube roof plate	Present		3G
TS18: forelimb bud	Present	Regionally restricted	3G	Expression is as a distally positioned stripe and as two weaker proximodistally expanded stripes.

Structure	Level	Pattern	Image	Note
TS18: dermomyotome	Present		3G'
TS18: hindbrain roof plate	Present		3G'
TS18: midbrain	Present		3G'
TS18: neural tube roof plate	Present		3G'
TS18: forelimb bud	Present	Regionally restricted	3G'	Expression is as a distally positioned stripe and as two weaker proximodistally expanded stripes.

Structure	Level	Pattern	Image	Note
TS18: dermomyotome	Present		3G''
TS18: hindbrain roof plate	Present		3G''
TS18: midbrain	Present		3G''
TS18: neural tube roof plate	Present		3G''
TS18: forelimb bud	Present	Regionally restricted	3G''	Expression is as a distally positioned stripe and as two weaker proximodistally expanded stripes.

Structure	Level	Pattern	Image	Note
TS18: dermomyotome	Present		3G'''
TS18: hindbrain roof plate	Present		3G'''
TS18: midbrain	Present		3G'''
TS18: neural tube roof plate	Present		3G'''
TS18: forelimb bud	Present	Regionally restricted	3G'''	Expression is as a distally positioned stripe and as two weaker proximodistally expanded stripes.
TS18: hindlimb bud	Present	Regionally restricted	3G'''	Expression is in the medial portion and in a more distal domain.

Structure	Level	Pattern	Image	Note
TS21: limb digit	Present		3H
TS21: midbrain	Present		3H
TS21: mystacial pad	Present		3H

Structure	Level	Pattern	Image	Note
TS21: hindlimb digit	Present		3H'
TS21: segmental spinal nerve	Present	Regionally restricted	3H'	Expression is in spinal nerve precursors.

Structure	Level	Pattern	Image	Note
TS21: forelimb digit	Present		3H''
TS21: radius cartilage condensation	Absent		3H''
TS21: radius-ulna cartilage condensation	Absent		3H''

Structure	Level	Pattern	Image	Note
TS21: hindlimb digit	Present		3H'''
TS21: tibia-fibula pre-cartilage condensation	Present		3H'''

Structure	Level	Pattern	Image	Note
TS15: 1st branchial arch	Present		4A
TS15: future hindbrain roof plate	Present		4A
TS15: neural tube roof plate	Present		4A

Structure	Level	Pattern	Image	Note
TS15: neural tube roof plate	Present		4B' , 4B''' , 4B
TS15: 1st branchial arch	Present		4B'' , 4B
TS15: dorsal root ganglion	Present		4B''' , 4B

Structure	Level	Pattern	Image	Note
TS17: dorsal root ganglion	Present		4C
TS17: hindbrain roof plate	Present		4C
TS17: neural tube roof plate	Present		4C

Structure	Level	Pattern	Image	Note
TS17: dorsal root ganglion	Present		4D , 4D'
TS17: neural tube roof plate	Present		4D , 4D'

Structure	Level	Pattern	Image	Note
TS17: dermomyotome	Present		4E , 4E'
TS17: dorsal root ganglion	Present		4E , 4E'

Structure	Level	Pattern	Image	Note
TS22: forelimb digit	Present	Regionally restricted	4F	Expression is in the cartilagenous regions between the phalanges corresponding to the joints.

Structure	Level	Pattern	Image	Note
TS22: hindlimb digit	Present	Regionally restricted	4G	Expression is in the cartilagenous regions between the phalanges corresponding to the joints.

Structure	Level	Pattern	Image	Note
TS22: forelimb digit	Present	Regionally restricted	4H	Expression is in the cartilagenous regions between the phalanges corresponding to the joints.
TS22: skeletal joint	Present	Regionally restricted	4H	Expression is in the cartilagenous regions between the phalanges corresponding to the joints.

Structure	Level	Pattern	Image	Note
TS22: forelimb digit	Present	Regionally restricted	4I	Expression is in the cartilagenous regions between the phalanges corresponding to the joints.
TS22: skeletal joint	Present	Regionally restricted	4I	Expression is in the cartilagenous regions between the phalanges corresponding to the joints.

Structure	Level	Pattern	Image	Note
TS22: hindlimb digit	Present	Regionally restricted	4J	Expression is in the cartilagenous regions between the phalanges corresponding to the joints.
TS22: skeletal joint	Present	Regionally restricted	4J	Expression is in the cartilagenous regions between the phalanges corresponding to the joints.

Structure	Level	Pattern	Image	Note
TS22: hindlimb digit	Present	Regionally restricted	4K	Expression is in the cartilagenous regions between the phalanges corresponding to the joints.
TS22: skeletal joint	Present	Regionally restricted	4K	Expression is in the cartilagenous regions between the phalanges corresponding to the joints.

Structure	Level	Pattern	Image	Note
TS22: forebrain ventricular layer	Strong		4L
TS22: midbrain ventricular layer	Strong		4L
TS22: spinal cord ventricular layer	Strong		4L
TS22: vibrissa follicle	Present		4L , 4L'''
TS22: dorsal root ganglion	Present		4L'' , 4L
TS22: inferior glossopharyngeal ganglion	Present		4L , 4L'
TS22: superior glossopharyngeal ganglion	Present		4L , 4L'
TS22: trigeminal ganglion	Present		4L , 4L'
TS22: vagus ganglion	Present		4L , 4L'
TS22: submandibular gland primordium	Present		4L , 4L''''
TS22: hyoid cartilage condensation	Present		4L
TS22: viscerocranium	Present	Regionally restricted	4L	Expression is in the turbinate bone.

Structure	Level	Pattern	Image	Note
TS15: tail unsegmented mesenchyme	Present	Regionally restricted	7A	Expression is in the presomitic mesoderm.

Structure	Level	Pattern	Image	Note
TS15: tail unsegmented mesenchyme	Present	Regionally restricted	7A'	Expression is in the presomitic mesoderm.

Structure	Level	Pattern	Image	Note
TS15: tail unsegmented mesenchyme	Present	Regionally restricted	7A''	Expression is in the presomitic mesoderm.

Structure	Level	Pattern	Image	Note
TS15: tail unsegmented mesenchyme	Present	Regionally restricted	7B	Expression is in the presomitic mesoderm.

Structure	Level	Pattern	Image	Note
TS15: tail unsegmented mesenchyme	Present	Regionally restricted	7B'	Expression is in the presomitic mesoderm.

Structure	Level	Pattern	Image	Note
TS15: tail unsegmented mesenchyme	Present	Regionally restricted	7C Fam181b	Expression is in the presomitic mesoderm.

Structure	Level	Pattern	Image	Note
TS15: tail unsegmented mesenchyme	Present	Regionally restricted	7C' Fam181b	Expression is in the presomitic mesoderm.

Structure	Level	Pattern	Image	Note
TS15: tail unsegmented mesenchyme	Present	Regionally restricted	7D Fam181b	Expression is in the presomitic mesoderm.

Structure	Level	Pattern	Image	Note
TS15: tail unsegmented mesenchyme	Present	Regionally restricted	7D' Fam181b	Expression is in the presomitic mesoderm.

Structure	Level	Pattern	Image	Note
TS15: tail unsegmented mesenchyme	Present	Regionally restricted	7E	Expression is in the presomitic mesoderm.
TS15: telencephalon	Present		7E

Structure	Level	Pattern	Image	Note
TS15: telencephalon	Present	Regionally restricted	7E'	Expression is dorsal.
TS15: tail unsegmented mesenchyme	Absent		7E'	Expression is absent in the presomitic mesoderm.

Structure	Level	Pattern	Image	Note
TS17: mesenchyme derived from lateral plate	Present	Regionally restricted	8A , 8A'	Expression is in lateral plate mesenchyme.
TS17: limb	Present		8A , 8A'
TS17: tail unsegmented mesenchyme	Present		8A , 8A'

Structure	Level	Pattern	Image	Note
TS17: mesenchyme derived from lateral plate	Present	Regionally restricted	8B' , 8B	Expression is absent in lateral plate mesenchyme.
TS17: limb	Present		8B' , 8B
TS17: tail unsegmented mesenchyme	Absent		8B' , 8B

Structure	Level	Pattern	Image	Note
TS17: mesenchyme derived from lateral plate	Present	Regionally restricted	8C , 8C'	Expression is in lateral plate mesenchyme.
TS17: limb	Present		8C , 8C'
TS17: tail unsegmented mesenchyme	Absent		8C , 8C'

Structure	Level	Pattern	Image	Note
TS15: embryo	Present		9C

Structure	Level	Pattern	Image	Note
TS15: embryo	Absent		9C'