Tg(Prnp/PRNP*ARR)FF8Pcg
Transgene Detail
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| Symbol: |
Tg(Prnp/PRNP*ARR)FF8Pcg |
| Name: |
transgene insertion FF8, Peter Griffiths |
| MGI ID: |
MGI:5779525 |
| Synonyms: |
Tg(OvPrP)FF8 |
| Transgene: |
Tg(Prnp/PRNP*ARR)FF8Pcg Location: unknown
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| Alliance: |
Tg(Prnp/PRNP*ARR)FF8Pcg page
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| Transgene Type: |
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Transgenic (Humanized sequence, Inserted expressed sequence) |
| Mutation: |
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Insertion
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Tg(Prnp/PRNP*ARR)FF8Pcg expresses
2 genes
Transgene expresses:
| Organism |
Expressed Gene |
Note |
| mouse |
Prnp (MGI:97769) |
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| sheep |
PRNP (493887) |
Mouse/sheep chimeric protein |
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Mutation details: The 12-kb transgene, derived from the pPrHG "half genomic" vector, contains the promoter and first exon of the mouse prion protein gene (Prnp), mouse exon 2 joined to a chimeric mouse/sheep exon 3, and the 3'-untranslated region (UTR) of the mouse gene. The chimeric exon contains nucleotides (nt) 1-117 of the mouse Prnp coding sequence, encoding amino acids (aa) 1-39, and nt 118-762 of the sheep PRNP coding sequence encoding aa 40-253 of the ARR prion protein variant, which has alanine, arginine and arginine, respectively, at ovine polymorphic amino acid positions 136, 154 and 171 (A136R154R171). Mice bearing this transgene express mouse/ovine Prnp/PRNPARR from the mouse Prnp promoter in brain and other tissues.
(J:32214, J:82809, J:232471)
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| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 3 strains available
Cell Lines: 0 lines available
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| Original: |
J:232471 Veterinary Laboratories Agency, Preparation of more sensitive bioassay models for the improved detection, differentiation and diagnosis of the BSE agent. SID 5 Research Project Final Report, DEFRA Project Code SE1753. 2009; |
| All: |
3 reference(s) |
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Analysis Tools
