Probe:	Sk-mE2F-5
Probe preparation:	labelled with P32 DNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS15: future forebrain	Present		5A	Expression first detected in forebrain.
TS15: future midbrain	Present		5A	Lower levels of expression in the midbrain.

Structure	Level	Pattern	Image	Note
TS17: hindbrain	Present		5B	Expression in midbrain and hindbrain.
TS17: midbrain	Present		5B	Expression in midbrain and hindbrain.
TS17: future spinal cord	Very strong	Regionally restricted	5B	Very strong expression in rostral, but not in caudal regions of spinal cord.

Structure	Level	Pattern	Image	Note
TS21: brain ventricular layer	Strong		5C/D	Peak expression at 12.5-13.5 dpc.
TS21: dorsal root ganglion	Ambiguous		5C/D	Authors report expression barely detected in peripheral nervous system, such as the dorsal root ganglion.

Structure	Level	Pattern	Image	Note
TS23: retina nuclear layer	Strong		7K/L	Expression is high in the undifferentiated retinoblast layer.
TS23: neural retina	Present	Regionally restricted	7K/L	No expression in differentiated ganglion cell layer.

Structure	Level	Pattern	Image	Note
TS21: choroid plexus	Present		8D

Structure	Level	Pattern	Image	Note
TS19: central nervous system	Present	Widespread		Expression throughout the developing central nervous system.
TS19: brain ventricular layer	Present
TS19: brain marginal layer	Absent
TS19: neural retina	Present	Regionally restricted		Expression in neuroblastic layer.
TS19: heart	Present
TS19: liver	Present

Structure	Level	Pattern	Image	Note
TS20: brain ventricular layer	Present	Widespread		Broad expression in undifferentiated ventricular regions of the brain.
TS20: brain marginal layer	Absent
TS20: spinal cord ventricular layer	Present
TS20: spinal cord marginal layer	Absent
TS20: dorsal root ganglion	Absent
TS20: lung	Present

Structure	Level	Pattern	Image	Note
TS21: choroid plexus	Strong	Regionally restricted		At 13.5 -18.5 dpc, high levels of expression in anterior and posterior choroid plexus epithelia.
TS21: metanephros	Present
TS21: cartilage condensation	Weak	Regionally restricted		Expression in chondrocytes.

Structure	Level	Pattern	Image	Note
TS22: neural retina	Strong	Regionally restricted		Very abundant expression in embryonic retinoblastic cell layer. No expression in differentiated ganglion cell layer.

Structure	Level	Pattern	Image	Note
TS23: brain ventricular layer	Present	Widespread		Broad expression in undifferentiated ventricular regions of the brain.
TS23: thymus primordium	Absent
TS23: skeletal muscle	Weak

Structure	Level	Pattern	Image	Note
TS25: brain	Weak			Very low expression in brain.

Structure	Level	Pattern	Image	Note
TS26: retina nuclear layer	Strong			Very abundant expression in embryonic retinoblastic cell layer.
TS26: neural retina	Present	Regionally restricted		No expression in differentiated ganglion cell layer.
TS26: spinal cord ventricular layer	Present
TS26: spinal cord marginal layer	Absent
TS26: dorsal root ganglion	Absent
TS26: choroid plexus	Strong	Regionally restricted		At 13.5 -18.5 dpc, high levels of expression in anterior and posterior choroid plexus epithelia.
TS26: bone	Absent			No expression in osteoblasts.

Structure	Level	Pattern	Image	Note
TS26: brain	Weak			Very low expression in brain.
TS26: brain ventricular layer	Present
TS26: brain marginal layer	Absent
TS26: bone	Absent			No expression in osteoblasts.