Probe:	mE2F-4 Pst
Probe preparation:	labelled with P32 DNA
Visualized with:	Autoradiography
Assay notes:	Two probes/antibodies were described, but the one used in this assay was not detailed. Probe/antibody name MGI:1097406 may have been used in this assay.

Structure	Level	Pattern	Image	Note
TS21: brain	Present	Widespread	6E/F	Highest level of expression between 11.5 and 13.5 dpc.
TS21: brain marginal layer	Present		6E/F	Levels of expression were similar in ventricular, intermediate and marginal regions.
TS21: brain ventricular layer	Present		6E/F	Levels of expression were similar in ventricular, intermediate and marginal regions.
TS21: thalamus	Present		6E/F

Structure	Level	Pattern	Image	Note
TS23: dorsal root ganglion	Strong		6G/H
TS23: neural retina	Present	Regionally restricted	6G/H

Structure	Level	Pattern	Image	Note
TS23: neural retina	Present	Regionally restricted	7I/J	Expression in retinoblastic and ganglion cell layers.

Structure	Level	Pattern	Image	Note
TS21: 4th ventricle choroid plexus	Present		8C	Expression in posterior choroid plexus.

Structure	Level	Pattern	Image	Note
TS19: central nervous system	Present	Widespread		Highest level of expression between 11.5 and 13.5 dpc.
TS19: peripheral nervous system	Present	Widespread		Highest level of expression between 11.5 and 13.5 dpc.
TS19: brain marginal layer	Present
TS19: brain ventricular layer	Present
TS19: neural retina	Present	Regionally restricted		Expression in neuroblastic layer.
TS19: liver	Strong
TS19: heart	Present

Structure	Level	Pattern	Image	Note
TS20: neural retina	Present	Regionally restricted		Expression in retinoblastic and ganglion cell layers.
TS20: dorsal root ganglion	Present
TS20: spinal cord marginal layer	Present
TS20: spinal cord ventricular layer	Present
TS20: lung	Present
TS20: rib pre-cartilage condensation	Strong	Regionally restricted		Between 12.5 and 19.5 dpc, high levels of expression in areas corresponding to prolferating chondrocytes.
TS20: vertebral cartilage condensation	Strong	Regionally restricted		Between 12.5 and 19.5 dpc, high levels of expression in areas corresponding to prolferating chondrocytes.

Structure	Level	Pattern	Image	Note
TS21: neural retina	Present	Regionally restricted		Expression in retinoblastic and ganglion cell layers.
TS21: choroid plexus	Strong	Regionally restricted		At 13.5 -18.5 dpc, high levels of expression in anterior and posterior choroid plexus epithelia.
TS21: metanephros	Present

Structure	Level	Pattern	Image	Note
TS22: neural retina	Present	Regionally restricted		Expression in neuroblaastic and gangion cell layers.

Structure	Level	Pattern	Image	Note
TS23: thymus primordium	Very strong			Very high levels of expression present from 15.5 to 19.5 dpc.
TS23: skeletal muscle	Weak

Structure	Level	Pattern	Image	Note
TS26: central nervous system	Present	Widespread
TS26: peripheral nervous system	Present	Widespread
TS26: spinal cord marginal layer	Present
TS26: spinal cord ventricular layer	Present
TS26: neural retina	Present	Regionally restricted		Expression in retinoblastic and ganglion cell layers.
TS26: dorsal root ganglion	Present
TS26: choroid plexus	Strong	Regionally restricted		At 13.5 -18.5 dpc, high levels of expression in anterior and posterior choroid plexus epithelia.
TS26: bone	Absent			No expression in osteoblasts.

Structure	Level	Pattern	Image	Note
TS26: brain marginal layer	Present
TS26: brain ventricular layer	Present
TS26: thymus	Very strong			Very high levels of expression present from 15.5 to 19.5 dpc.
TS26: rib cartilage	Strong			Between 12.5 and 19.5 dpc, high levels of expression in areas corresponding to proliferating chondrocytes.
TS26: vertebral cartilage condensation	Strong			Between 12.5 and 19.5 dpc, high levels of expression in areas corresponding to proliferating chondrocytes.
TS26: bone	Absent			No expression in osteoblasts.