Probe:	pAlter-mE2F-1
Probe preparation:	labelled with P32 DNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS15: central nervous system	Present		3A/B	Expression increases in neural tube.

Structure	Level	Pattern	Image	Note
TS17: forebrain	Present		3C	Expression increases throughout brain.
TS17: midbrain	Present		3C	Expression increases throughout brain.
TS17: hindbrain	Strong		3C	Highest levels of expression in the hindbrain.
TS17: future spinal cord	Weak	Regionally restricted	3C	Expression only in the rostral half.

Structure	Level	Pattern	Image	Note
TS25: cerebellum ventricular layer	Present		3D/E

Structure	Level	Pattern	Image	Note
TS23: retina nuclear layer	Present	Regionally restricted	7A/B	Expression confined to the undifferentiated retinoblastic layer.
TS23: neural retina	Present	Regionally restricted	7A/B	No expression in ganglion cell layer at any time.

Structure	Level	Pattern	Image	Note
TS27: retina nuclear layer	Present	Regionally restricted	7C/D	Expression continues in the retinoblastic layer.
TS27: retina ganglion cell layer	Absent		7C/D	No expression in ganglion cell layer at any time

Structure	Level	Pattern	Image	Note
TS28: retina inner nuclear layer	Absent		7E/F	Retinoblastic layer has differentiated and given rise to the inner and outer nuclear layers.
TS28: retina outer nuclear layer	Absent		7E/F	Retinoblastic layer has differentiated and given rise to the inner and outer nuclear layers.
TS28: retina ganglion cell layer	Absent		7E/F	No expression in ganglion cell layer at any time.

Structure	Level	Pattern	Image	Note
TS13: embryo	Trace	Widespread		Expression just above background throughout the entire embryo.

Structure	Level	Pattern	Image	Note
TS15: future forebrain	Present
TS15: future hindbrain	Absent
TS15: future midbrain	Absent
TS15: future spinal cord	Absent

Structure	Level	Pattern	Image	Note
TS19: brain	Present	Widespread		Expression throughout brain and spinal cord.
TS19: brain ventricular layer	Present
TS19: brain marginal layer	Absent			No expression in intermediate or marginal zone.
TS19: future spinal cord	Weak	Regionally restricted		Expression in spinal cord is scarce and uniformly distributed in ventral and dorsal regions.
TS19: neural retina	Present	Regionally restricted		Expression in neuroblastic layer.
TS19: liver	Strong
TS19: heart	Present

Structure	Level	Pattern	Image	Note
TS20: brain ventricular layer	Present	Widespread		Expression in ventricular regions throughout the brain.
TS20: spinal cord ventricular layer	Present
TS20: spinal cord marginal layer	Absent
TS20: dorsal root ganglion	Absent
TS20: lung	Present
TS20: cartilage condensation	Absent			No expression in chondrocytes.

Structure	Level	Pattern	Image	Note
TS21: brain ventricular layer	Strong			Expression peaks at 13.5 days of gestation.
TS21: choroid plexus	Absent
TS21: metanephros	Present

Structure	Level	Pattern	Image	Note
TS22: neural retina	Present	Regionally restricted		Expression in neuroblastic layer. No expression in ganglion cell layer.

Structure	Level	Pattern	Image	Note
TS23: thymus primordium	Weak
TS23: skeletal muscle	Absent

Structure	Level	Pattern	Image	Note
TS24: brain ventricular layer	Present	Widespread		Expression in ventricular regions throughout the brain.
TS24: spinal cord	Weak	Regionally restricted		Expression in spinal cord is scarce and uniformly distributed in ventral and dorsal regions.

Structure	Level	Pattern	Image	Note
TS25: brain	Weak			Expression negligible throughout the brain except for ventricular areas of cerebellum.

Structure	Level	Pattern	Image	Note
TS26: retina nuclear layer	Present	Regionally restricted		Expression in neuroblastic layer. No expression in ganglion cell layer.
TS26: dorsal root ganglion	Absent
TS26: spinal cord ventricular layer	Present
TS26: spinal cord marginal layer	Absent
TS26: choroid plexus	Absent
TS26: bone	Absent			No expression in osteoblasts.

Structure	Level	Pattern	Image	Note
TS26: brain ventricular layer	Present
TS26: brain marginal layer	Absent			No expression in intermediate or marginal zone.
TS26: choroid plexus	Absent
TS26: thymus	Weak
TS26: metanephros	Present
TS26: liver	Strong
TS26: heart	Present
TS26: lung	Present
TS26: skeletal muscle	Absent
TS26: cartilage condensation	Absent			No expression in chondrocytes