Summary | Mutation origin | Mutation description | Recombinase activity | Find Mice (IMSR) | References

Symbol:	En1tm1.1(dreo)Pjen
Name:	engrailed 1; targeted mutation 1.1, Patricia Jensen
MGI ID:	MGI:5776371
Synonyms:	En1Dre
Gene:	En1  Location: Chr1:120530246-120535719 bp, + strand  Genetic Position: Chr1, 52.74 cM, cytoband C2-qter
Alliance:	En1tm1.1(dreo)Pjen page

Germline Transmission:	Earliest citation of germline transmission: J:232241
Parent Cell Line:	G4 (ES Cell)
Strain of Origin:	(129S6/SvEvTac x C57BL/6NCrl)F1

Allele Type:    Targeted (Recombinase) Mutation:    Insertion   En1tm1.1(dreo)Pjen expression driven by 1 gene Knock-in expression driven by: Organism Driver Gene Note mouse En1 (MGI:95389)   Mutation details: The mammalian codon-optimized bacteriophage D6 site-specific recombinase gene (dreo) with a 5' a viral 2A oligopeptide (T2A) that mediates ribosomal skipping replaced the stop codon and 41 bp of the 3' UTR in exon 2 without altering the coding sequence. phiC31-mediated recombination removed the neomycin resistance cassette inserted downstream of the recombinase gene. (J:232241)

Activity:	Tissue activity of this recombinase allele
Driver:	En1 (mouse) Summary of all recombinase alleles driven by En1. Alliance mouse En1 gene page

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any En1 Mutation:	32 strains or lines available

Original:	J:232241 Plummer NW, et al., A knock-in allele of En1 expressing dre recombinase. Genesis. 2016 Aug;54(8):447-54
All:	3 reference(s)