Reporter:	GFP
	Direct Detection
Assay notes:	Heterozygous and homozygous embryos were indistinguishable.

Structure	Level	Pattern	Image	Note
TS3: 8-cell stage embryo	Absent			No expression was observed in the 7 embryos examined.

Structure	Level	Pattern	Image	Note
TS3: compacted morula	Present	Single cells	2A merge , 2A GFP	There are a few expressing cells; some of these cells are destined to form trophectoderm and some are destined to form inner cell mass.

Structure	Level	Pattern	Image	Note
TS4: inner cell mass	Present		2B merge , 2B GFP	In 12 of 30 early blastocyst stage embryos examined, expression is seen in both the inner cell mass and trophectoderm.
TS4: trophectoderm	Present		2B merge , 2B GFP	In 12 of 30 early blastocyst stage embryos examined, expression is seen in both the inner cell mass and trophectoderm.

Structure	Level	Pattern	Image	Note
TS4: inner cell mass	Present			In 18 of 30 early blastocyst stage embryos examined, expression is restricted to the inner cell mass.
TS4: trophectoderm	Absent			In 18 of 30 early blastocyst stage embryos examined, expression is restricted to the inner cell mass.

Structure	Level	Pattern	Image	Note
TS5: inner cell mass	Present	Non-Uniform	2C GFP , 2C merge	In the mid-blastocyst stage embryo, expression is distributed in the inner cell mass in an apparently random, heterogeneous manner.
TS5: trophectoderm	Absent		2C merge , 2C GFP

Structure	Level	Pattern	Image	Note
TS5: inner cell mass	Present	Graded	2D merge , 2D GFP	The majority of cells show expression. The strongest expression is restricted to cells lining the cavity, whereas cells exhibiting reduced levels of expression are located deeper within the inner cell mass.

Structure	Level	Pattern	Image	Note
TS6: primitive endoderm	Present		2E merge , 2E GFP	In the late blastocyst, expressing cells are found exclusively in the nascent primitive endoderm layer.

Structure	Level	Pattern	Image	Note
TS6: primitive endoderm	Present		2F GFP , 2F merge	Expression is restricted to cells derived from the primitive endoderm, the parietal and visceral endoderm.

Structure	Level	Pattern	Image	Note
TS7: extraembryonic visceral endoderm	Present		2G merge , 2G GFP	Expression is restricted to cells of the parietal and visceral endoderm.
TS7: parietal endoderm	Present		2G merge , 2G GFP	Expression is restricted to cells of the parietal and visceral endoderm.