Tg(Prnp/PRNP*AHQ)EF50Pcg
Transgene Detail
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| Symbol: |
Tg(Prnp/PRNP*AHQ)EF50Pcg |
| Name: |
transgene insertion EM50, Peter Griffiths |
| MGI ID: |
MGI:5776258 |
| Synonyms: |
EF50, Tg(Mu/OvPrPAHQ)EF50, Tg(OvPrP)EF50 |
| Transgene: |
Tg(Prnp/PRNP*AHQ)EF50Pcg Location: unknown
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| Alliance: |
Tg(Prnp/PRNP*AHQ)EF50Pcg page
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| Transgene Type: |
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Transgenic (Inserted expressed sequence) |
| Mutation: |
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Insertion
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Tg(Prnp/PRNP*AHQ)EF50Pcg expresses
1 gene
Transgene expresses:
| Organism |
Expressed Gene |
Note |
| sheep |
PRNP (493887) |
Mouse/sheep chimeric protein |
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Mutation details: The 12-kb transgene, derived from the pPrHG "half genomic" vector, contains the promoter and first exon of the mouse prion protein gene (Prnp), mouse exon 2 joined to a chimeric mouse/sheep exon 3, and the 3'-untranslated region (UTR) of the mouse gene. The chimeric exon contains nucleotides (nt) 1-117 of the mouse Prnp coding sequence, encoding amino acids (aa) 1-39, and nt 118-762 of the sheep PRNP coding sequence encoding aa 40-253 of the AHQ prion protein variant, which has alanine, histidine and glutamine at ovine polymorphic codons 136, 154 and 171 (A136H154Q171). This allele, while conferring relative resistance to infection of sheep with classic scrapie, is strongly associated with susceptibility to atypical scrapie. The transgene copy number of homozygous transgenic mice of line EF50 was estimated by Southern blot analysis to be 18.5 +/- 0.5. Expression of the chimeric protein in brain homogenates from homozygous transgenic mice of this line, estimated by western blot analysis using enhanced chemiluminescence and autoradiography for visualization and densitometric quantitation, is 2-4-fold that seen in normal ovine brainstem.
(J:32214, J:223991)
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| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 2 strains available
Cell Lines: 0 lines available
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| Original: |
J:223991 Griffiths PC, et al., Overexpression of chimaeric murine/ovine PrP (A136H154Q171) in transgenic mice facilitates transmission and differentiation of ruminant prions. J Gen Virol. 2013 Nov;94(Pt 11):2577-86 |
| All: |
2 reference(s) |
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Analysis Tools
