Gene Expression Data
Assay Details
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Assay
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Reference:
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J:65596
Voss AK, et al., Taube nuss is a novel gene essential for the survival of pluripotent cells of early mouse embryos. Development. 2000 Dec;127(24):5449-61
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:5775363
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Gene symbol:
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Pou5f1
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Gene name:
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POU domain, class 5, transcription factor 1
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Antibody:
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anti-Oct-4
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Detection system:
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Secondary antibody coupled to Alexa Fluor 568
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Assay notes:
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Stained embryos were the product of heterozygotic intercrosses. Based on other evidence, normal Mendelian ratios are to be expected at the ages examined. The genotype of individual embryos was not determined. Thus the genotype assigned to the pictured embryos is based on phenotypic criteria; in some cases more than one genotype exhibits the same phenotype.
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Results
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Specimen
8C:
(close  )
| Structure |
Level |
Pattern |
Image |
Note |
| TS4: blastocyst |
Present |
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8C
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No differences in staining levels were observed for any of the embryos at this age.
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Specimen
not shown - het:
(close )
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Genetic Background:
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involves: 129S2/SvPas
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Mutant Allele(s):
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Taf8Gt(pGT1.8geo)9Pgr/Taf8+
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Age:
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embryonic day 3.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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2% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS4: blastocyst |
Present |
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No differences in staining levels were observed for any of the embryos at this age.
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Specimen
not shown - WT:
embryonic day 3.5
(more  )
Specimen
not shown - WT:
(close )
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Genetic Background:
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involves: 129S2/SvPas
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Age:
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embryonic day 3.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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2% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS4: blastocyst |
Present |
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No differences in staining levels were observed for any of the embryos at this age.
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Specimen
8F:
(close )
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Genetic Background:
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involves: 129S2/SvPas
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Mutant Allele(s):
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Taf8Gt(pGT1.8geo)9Pgr/Taf8+
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Age:
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embryonic day 4.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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2% Paraformaldehyde
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Specimen
not shown - WT:
embryonic day 4.0
(more )
Specimen
not shown - WT:
(close )
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Genetic Background:
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involves: 129S2/SvPas
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Age:
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embryonic day 4.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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2% Paraformaldehyde
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Specimen
8I:
(close )
| Structure |
Level |
Pattern |
Image |
Note |
| TS5: inner cell mass |
Present |
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8I
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The inner cell mass is almost absent from the mutant embryos.
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Analysis Tools
