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Gene Expression Data
Assay Details
Assay
Reference: J:3129 Dong LJ, et al., The expression of the genes for entactin, laminin A, laminin B1 and laminin B2 in murine lens morphogenesis and eye development. Differentiation. 1991 Dec;48(3):157-72
Assay type: RNA in situ
MGI Accession ID: MGI:5774861
Gene symbol: Nid1
Gene name: nidogen 1
Probe: Entactin cDNA1
Probe preparation: Antisense labelled with S35 RNA
Visualized with: Autoradiography
Results
Specimen 2Aa: embryonic day 9.5-10.0 (more )
Note: 25 somites.
Structure Level Pattern Image Note
TS15: eye mesenchyme Strong Regionally restricted 2Aa Abundant staining was present in the mesodermal cells surrounding the optic vesicle.
TS15: lens placode Absent 2Aa
TS15: neural ectoderm Absent 2Aa

Specimen 2Bb: embryonic day 10.5 (more )
Note: 34 somites.
Structure Level Pattern Image Note
TS16: lens pit Present 2Bb
TS16: optic cup Absent 2Bb

Specimen 2Cc: embryonic day 10.5-11.0 (more )
Note: 38 somites.
Structure Level Pattern Image Note
TS17: optic cup Absent 2Cc
TS17: lens pit Strong Regionally restricted 2Cc Expression was localized primarily to the peripheral cells at the equator and anterior half of the lens.
TS17: hyaloid cavity Present Regionally restricted 2Cc Expression was detected in groups of cells thought to be hyalocytes in the vitreous of the eye.

Specimen 2Dd: embryonic day 15.5 (more )
Structure Level Pattern Image Note
TS23: vitreous humour Present Regionally restricted 2Dd Expression was detected in cells in a linear array adjacent to the posterior surface of the lens and the inner limiting membrane.
TS23: cornea epithelium Absent 2Dd
TS23: neural retina Absent 2Dd
TS23: eye mesenchyme Present 2Dd

Specimen 3A: embryonic day 9.5 (more )
Note: 25 somites.
Structure Level Pattern Image Note
TS15: lens placode Absent 3A
TS15: eye mesenchyme Present Regionally restricted 3A Expression was detected in mesodermal cells lying between the lens placode and the optic vesicle.

Specimen 3E: embryonic day 9.5 (more )
Note: 25 somites.
Structure Level Pattern Image Note
TS15: lens placode Absent 3E
TS15: eye mesenchyme Present Regionally restricted 3E Expression was detected in mesodermal cells lying between the lens placode and the optic vesicle.

Specimen 3F: embryonic day 9.5 (more )
Note: 25 somites.
Structure Level Pattern Image Note
TS15: eye mesenchyme Present Regionally restricted 3F Expression was detected in mesodermal cells lying between the lens placode and the optic vesicle.
TS15: head mesenchyme Strong Regionally restricted 3F Expression was heavily labeled in the basement membrane area of the diencephalon.

Specimen 4Aa: embryonic day 18.5 (more )
Structure Level Pattern Image Note
TS26: lens equatorial epithelium Strong 4Aa
TS26: vitreous humour Strong Regionally restricted 4Aa Expression was organized in a linear array adjacent to the posterior surface of the lens and by the inner limiting membrane.

Specimen 4Bb: postnatal day 0 (more )
Structure Level Pattern Image Note
TS27: lens equatorial epithelium Strong 4Bb
TS27: vitreous humour Strong Regionally restricted 4Bb Expression was organized in a linear array adjacent to the posterior surface of the lens and by the inner limiting membrane.
TS27: retina ganglion cell layer Absent 4Bb

Specimen 4Cc: postnatal day 7 (more )
Structure Level Pattern Image Note
TS28: lens equatorial epithelium Strong 4Cc
TS28: vitreous humour Strong Regionally restricted 4Cc Expression was organized in a linear array adjacent to the posterior surface of the lens and by the inner limiting membrane.
TS28: retina ganglion cell layer Absent 4Cc

Specimen 6A: embryonic day 10.5-11.0 (more )
Note: 38 somites.
Structure Level Pattern Image Note
TS17: optic cup outer layer Absent 6A
TS17: eye mesenchyme Present Regionally restricted 6A Expression was detected in mesenchymal cells close to the pigment epithelium.

Specimen 8A: postnatal newborn (more )
Structure Level Pattern Image Note
TS27: vitreous humour Present 8A Expression was detected in hyalocytes.
TS27: retina ganglion cell layer Absent 8A


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
04/23/2024
MGI 6.23
The Jackson Laboratory