Gene Expression Data
Assay Details
Assay
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Reference:
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J:3129
Dong LJ, et al., The expression of the genes for entactin, laminin A, laminin B1 and laminin B2 in murine lens morphogenesis and eye development. Differentiation. 1991 Dec;48(3):157-72
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:5774861
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Gene symbol:
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Nid1
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Gene name:
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nidogen 1
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Probe:
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Entactin cDNA1
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Probe preparation:
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Antisense labelled with S35 RNA
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Visualized with:
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Autoradiography
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Results
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Specimen
2Aa:
embryonic day 9.5-10.0
(more )
Note: 25 somites.
Specimen
2Aa:
(close )
Genetic Background:
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involves: C57BL/6 * CBA
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Age:
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embryonic day 9.5-10.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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25 somites.
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Specimen
2Bb:
embryonic day 10.5
(more )
Note: 34 somites.
Specimen
2Bb:
(close )
Genetic Background:
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involves: C57BL/6 * CBA
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
|
34 somites.
|
Specimen
2Cc:
embryonic day 10.5-11.0
(more )
Note: 38 somites.
Specimen
2Cc:
(close )
Genetic Background:
|
involves: C57BL/6 * CBA
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Age:
|
embryonic day 10.5-11.0
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Sex:
|
Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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38 somites.
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: optic cup |
Absent |
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2Cc
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TS17: lens pit |
Strong |
Regionally restricted |
2Cc
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Expression was localized primarily to the peripheral cells at the equator and anterior half of the lens.
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TS17: hyaloid cavity |
Present |
Regionally restricted |
2Cc
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Expression was detected in groups of cells thought to be hyalocytes in the vitreous of the eye.
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Specimen
2Dd:
embryonic day 15.5
(more )
Specimen
2Dd:
(close )
Genetic Background:
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involves: C57BL/6 * CBA
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Age:
|
embryonic day 15.5
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Sex:
|
Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
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Cryosection
|
Specimen
3A:
embryonic day 9.5
(more )
Note: 25 somites.
Specimen
3A:
(close )
Genetic Background:
|
involves: C57BL/6 * CBA
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Age:
|
embryonic day 9.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
|
25 somites.
|
Structure |
Level |
Pattern |
Image |
Note |
TS15: lens placode |
Absent |
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3A
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TS15: eye mesenchyme |
Present |
Regionally restricted |
3A
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Expression was detected in mesodermal cells lying between the lens placode and the optic vesicle.
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Specimen
3E:
embryonic day 9.5
(more )
Note: 25 somites.
Specimen
3E:
(close )
Genetic Background:
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involves: C57BL/6 * CBA
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Age:
|
embryonic day 9.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
|
Cryosection
|
Note:
|
25 somites.
|
Structure |
Level |
Pattern |
Image |
Note |
TS15: lens placode |
Absent |
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3E
|
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TS15: eye mesenchyme |
Present |
Regionally restricted |
3E
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Expression was detected in mesodermal cells lying between the lens placode and the optic vesicle.
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Specimen
3F:
embryonic day 9.5
(more )
Note: 25 somites.
Specimen
3F:
(close )
Genetic Background:
|
involves: C57BL/6 * CBA
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Age:
|
embryonic day 9.5
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
25 somites.
|
Structure |
Level |
Pattern |
Image |
Note |
TS15: eye mesenchyme |
Present |
Regionally restricted |
3F
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Expression was detected in mesodermal cells lying between the lens placode and the optic vesicle.
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TS15: head mesenchyme |
Strong |
Regionally restricted |
3F
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Expression was heavily labeled in the basement membrane area of the diencephalon.
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Specimen
4Aa:
embryonic day 18.5
(more )
Specimen
4Aa:
(close )
Genetic Background:
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involves: C57BL/6 * CBA
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Age:
|
embryonic day 18.5
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Sex:
|
Not Specified
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Type:
|
section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
4Bb:
postnatal day 0
(more )
Specimen
4Bb:
(close )
Genetic Background:
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involves: C57BL/6 * CBA
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Age:
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postnatal day 0
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Sex:
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Not Specified
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Type:
|
section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
4Cc:
postnatal day 7
(more )
Specimen
4Cc:
(close )
Genetic Background:
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involves: C57BL/6 * CBA
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Age:
|
postnatal day 7
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Sex:
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Not Specified
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Type:
|
section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
6A:
embryonic day 10.5-11.0
(more )
Note: 38 somites.
Specimen
6A:
(close )
Genetic Background:
|
involves: C57BL/6 * CBA
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Age:
|
embryonic day 10.5-11.0
|
Sex:
|
Not Specified
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Type:
|
section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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38 somites.
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Specimen
8A:
postnatal newborn
(more )
Specimen
8A:
(close )
Genetic Background:
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involves: C57BL/6 * CBA
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Age:
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postnatal newborn
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Sex:
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Not Specified
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Type:
|
section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
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Cryosection
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