Antibody:	Anti-Entactin
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS15: lens placode	Present	Regionally restricted	1A	Expression was detected in the region of the basal lamina.
TS15: optic vesicle	Present	Regionally restricted	1A	Expression was detected in the region of the basal lamina.
TS15: eye mesenchyme	Present	Regionally restricted	1A	Expression was detected in the mesoderm in the interspace between the lens placode and the optic vesicle.

Structure	Level	Pattern	Image	Note
TS16: lens pit	Present		1B
TS16: optic cup	Present		1B
TS16: eye	Present	Regionally restricted	1B	There was distinct staining of the connecting fibers in the interspace between the lens pit and optic cup.

Structure	Level	Pattern	Image	Note
TS20: lens vesicle	Present	Regionally restricted	1C	Expression was detected in the lens capsule.
TS20: retina internal limiting lamina	Present		1C

Structure	Level	Pattern	Image	Note
TS26: lens	Strong	Regionally restricted	1D	Heavy staining persisted in the lens capsule.
TS26: retina internal limiting lamina	Strong		1D	Heavy staining persisted.
TS26: vitreous humour	Strong		1D	Heavy staining persisted in hyalocytes.

Structure	Level	Pattern	Image	Note
TS19: lens vesicle	Present