Summary | Mutation origin | Mutation description | Find Mice (IMSR) | References

Symbol:	Gt(ROSA)26Sortm3.3(CAG-EGFP,-CHRM3*/mCherry/Htr2a)Pjen
Name:	gene trap ROSA 26, Philippe Soriano; targeted mutation 3.3, Patricia Jensen
MGI ID:	MGI:5771785
Synonyms:	RC::L-hM3Dq
Gene:	Gt(ROSA)26Sor  Location: Chr6:113044389-113054205 bp, - strand  Genetic Position: Chr6, 52.73 cM
Alliance:	Gt(ROSA)26Sortm3.3(CAG-EGFP,-CHRM3*/mCherry/Htr2a)Pjen page

Germline Transmission:	Earliest citation of germline transmission: J:235493
Parent Cell Line:	G4 (ES Cell)
Strain of Origin:	(129S6/SvEvTac x C57BL/6NCrl)F1

Allele Type:    Targeted (Conditional ready, Reporter) Mutation:    Insertion   Mutation details: The allele has a CAG hybrid promoter,a rox-flanked His3-SV40 transcriptional STOP cassette, an FRT-flanked STOP cassette and a variant lox-flanked region containing an eGFP cassette, an inverted hM3Dq/mCherry fusion protein, WPRE, polyA signal and an attB/attP-flanked PGK-frt-Neo-polyA cassette all inserted into the Gt(ROSA)26Sor locus. The inverted hM3Dq-mCherry-2ACT88 fusion protein cDNA sequence contains (hM3Dq DREADD) a red fluorescent protein mCherry and the final 264 coding nucleotides of the rat Htr2A gene (encoding a C-terminal epitope that mediates localization to the cell body/soma and dendrites). PhiC31-mediated recombination removed the AttB/AttP-flanked neo sequence. Dre-mediated recombination removed the rox-flanked STOP cassette. Flp-mediated recombination removed FRT-flanked STOP cassette. (J:235493)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Gt(ROSA)26Sor Mutation:	942 strains or lines available

Original:	J:235493 Sciolino NR, et al., Recombinase-Dependent Mouse Lines for Chemogenetic Activation of Genetically Defined Cell Types. Cell Rep. 2016 Jun 14;15(11):2563-73
All:	4 reference(s)