Antibody:	Anti-Tsp3
Detection system:	Secondary antibody coupled to fluorescein

Structure	Level	Pattern	Image	Note
TS12: amnion mesenchyme	Present		6A
TS12: extraembryonic endoderm	Absent		6A	Expression was absent from the endodermal layer underlying the extraembryonic mesoderm.

Structure	Level	Pattern	Image	Note
TS20: Meckel's cartilage	Present

Structure	Level	Pattern	Image	Note
TS24: skeletal muscle	Present

Structure	Level	Pattern	Image	Note
TS26: cartilage	Present	Regionally restricted	6B	Expression was detected in developing cartilage and in the region of calcified cartilage.
TS26: skeletal muscle	Present		6B
TS26: bone	Present		6B	Expression was in the forming bone.

Structure	Level	Pattern	Image	Note
TS26: retina layer	Present	Regionally restricted	6D	Expression was detected in the inner plexiform layer.
TS26: optic choroid	Present	Regionally restricted	6D	Expression was detected in Bruch's membrane.
TS26: lens fiber	Present	Widespread	6D
TS26: lens capsule	Absent		6D

Structure	Level	Pattern	Image	Note
TS22: peripheral nerve	Strong		6F
TS22: smooth muscle tissue	Present		6F

Structure	Level	Pattern	Image	Note
TS26: cerebellum Purkinje cell layer	Present		6G
TS26: cerebellum external granule cell layer	Present		6G
TS26: cerebellum white matter	Present		6G