Probe:	beta5 cDNA
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS17: embryo mesenchyme	Present	Ubiquitous	2A/B
TS17: neural tube	Absent		2A/B

Structure	Level	Pattern	Image	Note
TS22: yolk sac	Present	Diffuse	2C/D

Structure	Level	Pattern	Image	Note
TS23: heart atrium	Present		2E/F	Signals were always stronger than the myocardial signals.
TS23: cardiac muscle tissue	Present		2E/F

Structure	Level	Pattern	Image	Note
TS25: aorta	Present	Regionally restricted	3A/B	Expressed in the endothelial cells and their surrounding mesenchyme.

Structure	Level	Pattern	Image	Note
TS22: choroidal vasculature	Strong		3C/D

Structure	Level	Pattern	Image	Note
TS23: choroidal vasculature	Strong		3E/F

Structure	Level	Pattern	Image	Note
TS22: bronchiole	Strong	Regionally restricted	3G/H	Expressed in the mesenchyme immediately surrounding the bronchioli. No expression in the respiratory epithelium of the bronchioli.

Structure	Level	Pattern	Image	Note
TS25: vertebra	Strong	Regionally restricted	4A/B	Expressed along the surface and in the mesenchyme surrounding the nucleus pulpeosus but not within the ossified and the ossifying parts.

Structure	Level	Pattern	Image	Note
TS22: rib cartilage condensation	Present		4C/D	Expressed in some of the mesenchyme surrounding the developing ribs, which will eventually become the intercostal ligaments.

Structure	Level	Pattern	Image	Note
TS24: trachea mesenchyme	Present	Regionally restricted	4E/F	Expressed in the mesenchyme surrounding the tracheal epithelium, the smooth muscle cells of the tracheal wall, and the developing tracheal cartilages.
TS24: trachea cartilaginous ring	Present		4E/F

Structure	Level	Pattern	Image	Note
TS20: nose	Present		4G/H	Expressed in olfactive structures.

Structure	Level	Pattern	Image	Note
TS22: maturing nephron	Strong	Regionally restricted	5A/B	Expressed in the podocytes of the nephrogenic zone.

Structure	Level	Pattern	Image	Note
TS23: adrenal medulla	Present	Diffuse	5C/D

Structure	Level	Pattern	Image	Note
TS26: intestine epithelium	Present		5E/F

Structure	Level	Pattern	Image	Note
TS21: brain	Present	Regionally restricted	5G/H	Expressed throughout the ependymal layer lining the cerebrospinal fluid.

Structure	Level	Pattern	Image	Note
TS22: cranium	Strong		6A	Expressed in the superficial developing cartilages of the face and skull.
TS22: tooth	Present		6A
TS22: neural retinal epithelium	Absent		6A
TS22: pigmented retinal epithelium	Absent		6A

Structure	Level	Pattern	Image	Note
TS25: vertebra	Strong	Regionally restricted	6B	Expressed along the surface.
TS25: cranium	Strong		6B	Expressed in the superficial developing cartilages of the face and skull.
TS25: neural retina	Absent		6B
TS25: pigmented retinal epithelium	Absent		6B

Structure	Level	Pattern	Image	Note
TS25: vertebra	Strong	Regionally restricted	6C	Expressed along the surface.
TS25: cranium	Strong		6C	Expressed in the superficial developing cartilages of the face and skull.
TS25: neural retina	Absent		6C
TS25: pigmented retinal epithelium	Absent		6C

Structure	Level	Pattern	Image	Note
TS26: brain meninges	Present		6D
TS26: optic choroid	Present		6D
TS26: spinal cord meninges	Present		6D
TS26: neural retina	Absent		6D
TS26: pigmented retinal epithelium	Absent		6D

Structure	Level	Pattern	Image	Note
TS15: embryo mesenchyme	Present	Ubiquitous
TS15: 1st branchial arch mandibular component mesenchyme	Present
TS15: somite	Present
TS15: central nervous system	Absent
TS15: embryo ectoderm	Absent
TS15: embryo endoderm	Absent

Structure	Level	Pattern	Image	Note
TS17: 1st branchial arch mandibular component mesenchyme	Present
TS17: blood vessel	Present
TS17: somite	Present
TS17: central nervous system	Absent
TS17: embryo ectoderm	Absent
TS17: embryo endoderm	Absent
TS17: embryo mesenchyme	Present	Ubiquitous
TS17: trunk mesenchyme	Present	Regionally restricted		Expressed in the mesenchyme of the mesenterium and in the mesenchyme underlying the ectodermal epithelium.
TS17: surface ectoderm	Moderate			Expression in the developing cutaneous epithelium.

Structure	Level	Pattern	Image	Note
TS19: yolk sac	Present	Diffuse
TS19: somite	Present	Regionally restricted		Especially to their sclerodermal component.
TS19: brain mantle layer	Absent
TS19: brain marginal layer	Absent

Structure	Level	Pattern	Image	Note
TS20: yolk sac	Present	Diffuse
TS20: heart	Present			Expressed in the developing heart, especially at its periphery.
TS20: rib pre-cartilage condensation	Present
TS20: central nervous system	Present	Regionally restricted		Expressed throughout the ependymal layer lining the cerebrospinal fluid containing cavities.
TS20: midbrain floor plate	Present	Regionally restricted		Expressed in neurons surrounding the ependymal layer.

Structure	Level	Pattern	Image	Note
TS21: visceral pericardium	Strong			epicardium
TS21: cardiac muscle tissue	Strong	Regionally restricted		Abundent expression in the outer myocardial layer. Moderate expression in deeper myocardial layers in the myocardium and endocardium.
TS21: endocardial tissue	Moderate
TS21: vertebral cartilage condensation	Present	Regionally restricted		Expressed in the perichondral mesenchyme surrounding the developing vertebrae. No expression in the core of the vertebral bodies.
TS21: renal corpuscle	Strong	Regionally restricted		Expressed in glomerular epithelial cells.
TS21: mesenchyme of metanephros	Weak	Diffuse
TS21: ureteral vasculature	Weak	Diffuse
TS21: urothelium of ureter	Weak	Diffuse
TS21: adrenal gland	Present	Regionally restricted		Expressed in adrenal medulla.
TS21: pituitary gland	Present	Regionally restricted		Expressed in the mesenchyme.
TS21: remnant of Rathke's pouch	Present	Regionally restricted		Expressed in the mesenchyme.

Structure	Level	Pattern	Image	Note
TS22: central nervous system	Trace			Near absence.
TS22: liver	Absent
TS22: trachea mesenchyme	Present	Regionally restricted		Expressed in the mesenchyme surrounding the tracheal epithelium and the developing tracheal cartilages.
TS22: main olfactory bulb	Strong	Regionally restricted		Expressed in the cartilages that will form the bony walls of the olfactive structures, both within their intracartilagenous structure and in their surrounding mesenchyme.
TS22: pituitary gland	Present	Regionally restricted		Expressed in the mesenchyme.
TS22: remnant of Rathke's pouch	Present	Regionally restricted		Expressed in the mesenchyme.

Structure	Level	Pattern	Image	Note
TS23: vertebra	Present			Expressed in the segmented axial skeletal.
TS23: limb pre-cartilage condensation	Present
TS23: brain	Present	Regionally restricted		Expressed throughout the ependymal layer lining the cerebrospinal fluid.

Structure	Level	Pattern	Image	Note
TS24: lung vascular element	Present	Regionally restricted		Expressed in the endothelial cells. No expression in the mesenchyme surrounding the walls of the medium and large vessels.
TS24: bronchiole	Trace	Regionally restricted		Expression in the mesenchyme surrounding the bronchioli was almost undetectable.
TS24: central nervous system	Present	Regionally restricted		Expressed throughout the ependymal layer lining the cerebrospinal fluid containing cavities.

Structure	Level	Pattern	Image	Note
TS25: cardiac muscle tissue	Present
TS25: midbrain floor plate	Present	Regionally restricted		Expressed in neurons surrounding the ependymal layer.

Structure	Level	Pattern	Image	Note
TS26: vertebra	Strong	Regionally restricted		Expressed in the perichondral mesenchyme surrounding the developing vertebrae. No expression in the core of the vertebral bodies. Strong expression along the surface and in the mesenchyme surrounding the nucleus pulpeosus.

Structure	Level	Pattern	Image	Note
TS26: cardiac muscle tissue	Present

Structure	Level	Pattern	Image	Note
TS27: heart atrium	Present			Signals were always stronger than the myocardial signals.
TS27: cardiac muscle tissue	Present