Probe:	Gsh-4 cDNA3
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS19: neural tube	Present	Regionally restricted	4a	Expressed in the developing neural tube throughout the caudal half of the embryo.

Structure	Level	Pattern	Image	Note
TS20: hindbrain	Strong	Regionally restricted	4b	Expressed as an intense band of signal that coursed along the ventral aspect of the fourth ventricle to the cerebral aqueduct where it ended before reaching the region of the third ventricle.
TS20: midbrain	Strong	Regionally restricted	4b	Expressed as an intense band of signal that coursed along the ventral aspect of the fourth ventricle to the cerebral aqueduct where it ended before reaching the region of the third ventricle.

Structure	Level	Pattern	Image	Note
TS20: spinal cord	Present		4c

Structure	Level	Pattern	Image	Note
TS17: neural tube	Present	Regionally restricted	4d	Expressed in two symmetric regions localized to the ventral lateral aspects of the developing neural tube, confined to a subset of primitive neuroepithelial cells.

Structure	Level	Pattern	Image	Note
TS20: midbrain	Present	Regionally restricted	4e/f	Expressed in symmetric regions in the primitive neuroepithelial cells.

Structure	Level	Pattern	Image	Note
TS20: midbrain	Present	Regionally restricted	4g	These two bands of expression appeared to fuse together at the most rostral point in the ventral aspect of the cerebral aqueduct.

Structure	Level	Pattern	Image	Note
TS16: neural tube	Present	Regionally restricted	4h	Expressed as two dramatic stripes running from the brain to the end of the tail.

Structure	Level	Pattern	Image	Note
TS19: neural tube	Present	Scattered	4i	Expressed in a speckled pattern on the flanks of the developing spinal cord, just dorsal to the adjacent somites.

Structure	Level	Pattern	Image	Note
TS13: embryo	Absent

Structure	Level	Pattern	Image	Note
TS15: embryo	Present
TS15: inner ear	Absent
TS15: peripheral nervous system	Absent

Structure	Level	Pattern	Image	Note
TS16: neural tube	Present	Regionally restricted		Expressing regions corresponded to the ventrolateral domains of the developing spinal cord.

Structure	Level	Pattern	Image	Note
TS19: Rathke's pouch	Absent
TS19: inner ear	Absent
TS19: peripheral nervous system	Absent
TS19: retina	Absent

Structure	Level	Pattern	Image	Note
TS20: inner ear	Absent
TS20: peripheral nervous system	Absent
TS20: remnant of Rathke's pouch	Absent
TS20: retina	Absent
TS20: basal columns	Present

Structure	Level	Pattern	Image	Note
TS21: embryo	Absent

Structure	Level	Pattern	Image	Note
TS23: brain	Absent
TS23: spinal cord	Absent

Structure	Level	Pattern	Image	Note
TS27: mouse	Absent