Summary | Transgene origin | Transgene description | Find Mice (IMSR) | References

Symbol:	Tg(Ins2-Chrm3*)SJwe
Name:	transgene insertion S, Jurgen Wess
MGI ID:	MGI:5766547
Synonyms:	beta-R-s Tg
Transgene:	Tg(Ins2-Chrm3*)SJwe  Location: unknown
Alliance:	Tg(Ins2-Chrm3*)SJwe page

Strain of Origin:

C57BL/6NTac

Transgene Type:    Transgenic (Inserted expressed sequence) Mutation:    Insertion   Tg(Ins2-Chrm3*)SJwe expresses 1 gene Transgene expresses: Organism Expressed Gene Note Not Specified Chrm3 rat/turkey fusion   Tg(Ins2-Chrm3*)SJwe expression driven by 1 gene Transgene expression driven by: Organism Driver Gene Homolog in Mouse Note rat Ins2 (24506) Ins2  (MGI:96573)   Mutation details: The transgene was designed to have (from 5' to 3') a 650 bp rat insulin 2 (Ins2; RIP II) promoter fragment, a hemagglutinin epitope tag (HA), the GsD coding sequence (R-s; described below), and untranslated human growth hormone sequences (containing transcriptional termination, polyadenylation and splicing signals). The GsD sequence is a Galpha>s<-coupled rat M3 DREADD (designer receptor exclusively activated by designer drug). To create the rM3Ds sequence, the wildtype rat muscarinic 3 receptor (Chrm3; M3R) sequence was first modified to replace the second and third intracellular loops with the corresponding turkey beta1-adrenergic receptor (ADRB1 or beta1AR) sequences. The second modification introduced, via site-directed mutagenesis, the Y148C and A238G amino acid substitutions that abolish receptor affinity for the native ligand, acetylcholine (ACh), but allow receptor binding and subsequent activation by the small drug-like molecule clozapine-N-oxide (CNO). (J:154657)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available

Original:	J:154657 Guettier JM, et al., A chemical-genetic approach to study G protein regulation of beta cell function in vivo. Proc Natl Acad Sci U S A. 2009 Nov 10;106(45):19197-202
All:	1 reference(s)