Summary | Transgene origin | Transgene description | Find Mice (IMSR) | References

Symbol:	Tg(Ins2-Chrm3*)QJwe
Name:	transgene insertion Q, Jurgen Wess
MGI ID:	MGI:5766541
Synonyms:	beta-R-q Tg
Transgene:	Tg(Ins2-Chrm3*)QJwe  Location: unknown
Alliance:	Tg(Ins2-Chrm3*)QJwe page

Strain of Origin:

C57BL/6NTac

Transgene Type:    Transgenic (Inserted expressed sequence) Mutation:    Insertion   Tg(Ins2-Chrm3*)QJwe expresses 1 gene Transgene expresses: Organism Expressed Gene Note Not Specified Chrm3 rat/human fusion   Tg(Ins2-Chrm3*)QJwe expression driven by 1 gene Transgene expression driven by: Organism Driver Gene Homolog in Mouse Note rat Ins2 (24506) Ins2  (MGI:96573)   Mutation details: This transgene was designed to have (from 5' to 3') a 650 bp rat insulin 2 (Ins2; RIP II) promoter fragment, a hemagglutinin epitope tag (HA), the rhM3Dq coding sequence (R-q; described below), and untranslated human growth hormone sequences (containing transcriptional termination, polyadenylation and splicing signals). The transgene is expressed in pancreatic beta cells.The rhM3Dq (or R-q) sequence is a Gq-coupled rat/human chimer M3 muscarinic DREADD (designer receptor exclusively activated by designer drug). To create the rhM3Dq sequence, a 924 bp sequence of the wildtype rat muscarinic 3 receptor (Chrm3; M3R) was replaced with the human M3R sequences containing the Y149C and A239G amino acid substitutions (corresponding to rat Y148C and A238G). These mutations abolish receptor affinity for the native ligand, acetylcholine (ACh), but allow receptor binding and subsequent activation by the small drug-like molecule clozapine-N-oxide (CNO). (J:154657)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available

Original:	J:154657 Guettier JM, et al., A chemical-genetic approach to study G protein regulation of beta cell function in vivo. Proc Natl Acad Sci U S A. 2009 Nov 10;106(45):19197-202
All:	1 reference(s)