Gene Expression Data
Assay Details
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Assay
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Reference:
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J:165972
Wu X, et al., EMG1 is essential for mouse pre-implantation embryo development. BMC Dev Biol. 2010;10:99
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Assay type:
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In situ reporter (knock in)
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MGI Accession ID:
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MGI:5765967
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Gene symbol:
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Emg1
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Gene name:
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EMG1 N1-specific pseudouridine methyltransferase
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Reporter:
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lacZ
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Direct Detection
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Results
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Specimen
1D left zygote:
embryonic day 0.5; Emg1tm1Hdin/Emg1 +
(more  )
Note: The cross is between wild-type male and Emg1+/- female.
Specimen
1D left zygote:
(close  )
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Genetic Background:
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involves: 129S * 129S2/SvPas * 129X1/SvJ * CD-1 * ICR
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Mutant Allele(s):
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Emg1tm1Hdin/Emg1+
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Age:
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embryonic day 0.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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The cross is between wild-type male and Emg1+/- female.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS1: 1-cell stage embryo |
Present |
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1D left zygote
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Not present in those from breeding Emg1+/- males with wild-type females, indicating maternally transmitted EMG1.
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Specimen
1E +/- morula:
embryonic day 2.5; Emg1tm1Hdin/Emg1 +
(more )
Note: 8-cell morula. The cross is between wild-type male and Emg1+/- female.
Specimen
1E +/- morula:
(close )
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Genetic Background:
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involves: 129S * 129S2/SvPas * 129X1/SvJ * CD-1 * ICR
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Mutant Allele(s):
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Emg1tm1Hdin/Emg1+
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Age:
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embryonic day 2.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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8-cell morula. The cross is between wild-type male and Emg1+/- female.
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Specimen
1E +/- blastocyst:
embryonic day 3.5; Emg1tm1Hdin/Emg1 +
(more )
Note: The cross is between wild-type male and Emg1+/- female.
Specimen
1E +/- blastocyst:
(close )
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Genetic Background:
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involves: 129S * 129S2/SvPas * 129X1/SvJ * CD-1 * ICR
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Mutant Allele(s):
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Emg1tm1Hdin/Emg1+
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Age:
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embryonic day 3.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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The cross is between wild-type male and Emg1+/- female.
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Specimen
3D left:
embryonic day 8.5; Emg1tm1Hdin/Emg1 +
(more )
Specimen
3D left:
(close )
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Genetic Background:
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involves: 129S * 129S2/SvPas * 129X1/SvJ * CD-1 * ICR
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Mutant Allele(s):
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Emg1tm1Hdin/Emg1+
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
3D right:
embryonic day 9.0; Emg1tm1Hdin/Emg1 +
(more )
Specimen
3D right:
(close )
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Genetic Background:
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involves: 129S * 129S2/SvPas * 129X1/SvJ * CD-1 * ICR
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Mutant Allele(s):
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Emg1tm1Hdin/Emg1+
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Age:
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embryonic day 9.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Analysis Tools