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Gene Expression Data
Assay Details
Assay
Reference: J:147050 Nishioka N, et al., The Hippo signaling pathway components Lats and Yap pattern Tead4 activity to distinguish mouse trophectoderm from inner cell mass. Dev Cell. 2009 Mar;16(3):398-410
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5763604
Gene symbol: Cdx2
Gene name: caudal type homeobox 2
Antibody: anti-Cdx2 (CDX2-88)
Detection system: Secondary antibody coupled to Alexa Fluor 594
Results
Specimen 3B 4: embryonic day 2.0 (more )
Note: 4-cell. Double labeled: red - Cdx2; green - Yap1 (assay MGI:5763603).
Structure Level Pattern Image Note
TS3: 4-cell stage embryo Absent 3B Cdx2 4 , 3B merge 4
Specimen 3B 8: embryonic day 2.5 (more )
Note: 8-cell. Double labeled: red - Cdx2; green - Yap1 (assay MGI:5763603).
Structure Level Pattern Image Note
TS3: 8-cell stage embryo Present 3B merge 8 , 3B Cdx2 8
Specimen 3B 16: embryonic day 3.0 (more )
Note: 16-cell. Double labeled: red - Cdx2; green - Yap1 (assay MGI:5763603).
Structure Level Pattern Image Note
TS3: compacted morula Present 3B merge 16 , 3B Cdx2 16 Expression was detected in outside and inside cells.
Specimen 3B 30: embryonic day 3.0 (more )
Note: 30-cell. Double labeled: red - Cdx2; green - Yap1 (assay MGI:5763603).
Structure Level Pattern Image Note
TS4: compacted morula Present Non-Uniform 3B Cdx2 30 , 3B merge 30 Expression was detected in outside and inside cells.
Specimen 3B 34: embryonic day 3.5 (more )
Note: 34-cell. Double labeled: red - Cdx2; green - Yap1 (assay MGI:5763603).
Structure Level Pattern Image Note
TS4: blastocyst Present Non-Uniform 3B Cdx2 34 , 3B merge 34 Expression was not restricted to outside cells. Expression was detected in some inside cells.
Specimen 3C: embryonic day 3.5 (more )
Note: 38-cell. Double labeled: red - Cdx2; green - Yap1 (assay MGI:5763603).
Structure Level Pattern Image Note
TS4: trophectoderm Present 3C merge , 3C Cdx2 There was colocalization with Yap1 in trophectoderm cells.
Specimen 3E 32: embryonic day 3.5 (more )
Note: 32-cell. Double labeled: red - Cdx2; green - Wwtr1 (assay MGI:5763606).
Structure Level Pattern Image Note
TS4: compacted morula Present 3E merge 32 , 3E Cdx2 32
Specimen 3E 40: embryonic day 3.5 (more )
Note: 40-cell. Double labeled: red - Cdx2; green - Wwtr1 (assay MGI:5763606).
Structure Level Pattern Image Note
TS4: blastocyst Present 3E merge 40 , 3E Cdx2 40
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory