Gene Expression Data
Assay Details
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Assay
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Reference:
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J:147050
Nishioka N, et al., The Hippo signaling pathway components Lats and Yap pattern Tead4 activity to distinguish mouse trophectoderm from inner cell mass. Dev Cell. 2009 Mar;16(3):398-410
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:5763603
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Gene symbol:
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Yap1
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Gene name:
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yes-associated protein 1
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Antibody:
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Anti-Yap1
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Detection system:
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Secondary antibody coupled to Alexa Fluor 488
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Assay notes:
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Two antibodies were described - MGI:5763426 and MGI:5644471. The one used in this assay was not specified. Authors indicate that the two antibodies gave essentially the same results.
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Results
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Specimen
3B 4:
embryonic day 2.0
(more  )
Note: 4-cell. Double labeled: green - Yap1; red - Cdx2 (assay MGI:5763604).
Specimen
3B 4:
(close  )
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Genetic Background:
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involves: C57BL/6 * DBA
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Age:
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embryonic day 2.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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4-cell. Double labeled: green - Yap1; red - Cdx2 (assay MGI:5763604).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS3: 4-cell stage embryo |
Weak |
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3B merge 4
,
3B Yap 4
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Signal was weak and variable among embryos and among individual blastomeres.
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Specimen
3B 8:
embryonic day 2.5
(more )
Note: 8-cell. Double labeled: green - Yap1; red - Cdx2 (assay MGI:5763604).
Specimen
3B 8:
(close )
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Genetic Background:
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involves: C57BL/6 * DBA
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Age:
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embryonic day 2.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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8-cell. Double labeled: green - Yap1; red - Cdx2 (assay MGI:5763604).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS3: 8-cell stage embryo |
Present |
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3B Yap 8
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3B merge 8
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Expression was detected in all blastomeres.
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Specimen
3B 16:
embryonic day 3.0
(more )
Note: 16-cell. Double labeled: green - Yap1; red - Cdx2 (assay MGI:5763604).
Specimen
3B 16:
(close )
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Genetic Background:
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involves: C57BL/6 * DBA
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Age:
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embryonic day 3.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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16-cell. Double labeled: green - Yap1; red - Cdx2 (assay MGI:5763604).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS3: compacted morula |
Present |
Non-Uniform |
3B Yap 16
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3B merge 16
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Levels of nuclear Yap1 increased in outside cells, whereas levels decreased in inside cells.
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Specimen
3B 30:
embryonic day 3.0
(more )
Note: 30-cell. Double labeled: green - Yap1; red - Cdx2 (assay MGI:5763604).
Specimen
3B 30:
(close )
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Genetic Background:
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involves: C57BL/6 * DBA
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Age:
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embryonic day 3.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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30-cell. Double labeled: green - Yap1; red - Cdx2 (assay MGI:5763604).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS4: compacted morula |
Present |
Non-Uniform |
3B merge 30
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3B Yap 30
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Levels of nuclear Yap1 increased in outside cells, whereas levels decreased in inside cells.
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Specimen
3B 34:
embryonic day 3.5
(more )
Note: 34-cell. Double labeled: green - Yap1; red - Cdx2 (assay MGI:5763604).
Specimen
3B 34:
(close )
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Genetic Background:
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involves: C57BL/6 * DBA
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Age:
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embryonic day 3.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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34-cell. Double labeled: green - Yap1; red - Cdx2 (assay MGI:5763604).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS4: blastocyst |
Present |
Non-Uniform |
3B merge 34
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3B Yap 34
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Increased staining in outside cells remained constant.
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Specimen
3C:
embryonic day 3.5
(more )
Note: 38-cell. Double labeled: green - Yap1; red - Cdx2 (assay MGI:5763604).
Specimen
3C:
(close )
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Genetic Background:
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involves: C57BL/6 * DBA
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Age:
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embryonic day 3.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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38-cell. Double labeled: green - Yap1; red - Cdx2 (assay MGI:5763604).
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Analysis Tools