Summary | Transgene origin | Transgene description | Recombinase activity | Find Mice (IMSR) | References

Symbol:	Tg(Opalin-icre/ERT2)#Rjfl
Name:	transgene insertion, Robin JM Franklin
MGI ID:	MGI:5763107
Synonyms:	Opalin-iCreERT2
Transgene:	Tg(Opalin-icre/ERT2)#Rjfl  Location: unknown
Alliance:	Tg(Opalin-icre/ERT2)#Rjfl page

Strain of Origin:

(C57BL/6 x CBA)F1

Transgene Type:    Transgenic (Inducible, Recombinase) Inducer:    tamoxifen Mutation:    Insertion   Tg(Opalin-icre/ERT2)#Rjfl expression driven by 1 gene Transgene expression driven by: Organism Driver Gene Note mouse Opalin (MGI:2657025)   Mutation details: The mouse was generated via bacterial artificial chromosome transgenesis using the Opalin genomic bacterial artificial chromosome RP24-510M10. Insertion of the iCreERT2-polyA-frt-Kanamycin-frt cassette was achieved by homologous recombination (with no loss of the genomic bacterial artificial chromosome) in BAC (RPCI24-510M10) immediately after the Opalin initiation codon ATG of the open reading frame of the Opalin sequence. The frt-kanamycin-frt was removed using flippase recombinase. Four founders were generated, three of which expressed the transgene. The one with highest expression was chosen for further experimentation. Line numbers were not indicated. The pound symbol (#) is used when no line is specified and/or lines are pooled. (J:229987)

Activity:	Tissue activity of this recombinase allele
Driver:	Opalin (mouse) Summary of all recombinase alleles driven by Opalin. Comparative matrix view of recombinase activities Alliance mouse Opalin gene page

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 0 strains available      Cell Lines: 0 lines available

Original:	J:229987 Crawford AH, et al., Pre-Existing Mature Oligodendrocytes Do Not Contribute to Remyelination following Toxin-Induced Spinal Cord Demyelination. Am J Pathol. 2016 Mar;186(3):511-6
All:	3 reference(s)