Probe:	c-fms probe
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS17: branchial arch	Present		1A
TS17: head	Present		1A
TS17: embryo	Present	Spotted	1A	Expressed in a very extensive speckled pattern throughout the embryo. Labeled cells are particularly concentrated in the head, around the branchial arches, and along the dorsal midline.

Structure	Level	Pattern	Image	Note
TS18: liver	Present		1B
TS18: embryo	Present	Spotted	1B	Expressed in a fine speckled pattern of labeled cells throughout the embryo.

Structure	Level	Pattern	Image	Note
TS20: foot epithelium	Present	Regionally restricted	1C	Expressed at the apical ectodermal ridge.
TS20: hindlimb interdigital region	Present	Regionally restricted	1C	Expressed in between the digits.

Structure	Level	Pattern	Image	Note
TS20: digit epithelium	Present	Regionally restricted	1D	Positive cells leaved the marginal sinus and infiltrated the mesenchyme.
TS20: digit mesenchyme	Present	Regionally restricted	1D	Positive cells leaved the marginal sinus and infiltrated the mesenchyme.

Structure	Level	Pattern	Image	Note
TS20: diencephalon ventricular layer	Present	Regionally restricted	1E	Expressed in cells lining the ventricular surface.
TS20: hindbrain ventricular layer	Present	Regionally restricted	1E	Expressed in cells lining the ventricular surface.
TS20: midbrain ventricular layer	Present	Regionally restricted	1E	Expressed in cells lining the ventricular surface.
TS20: telencephalon ventricular layer	Present	Regionally restricted	1E	Expressed in cells lining the ventricular surface.

Structure	Level	Pattern	Image	Note
TS19: body wall	Present	Regionally restricted	1F	Expressed along the dorsal midline, closely associated with the vascular spaces adjacent to the neural tube, within the premuscle mass and the epidermis.

Structure	Level	Pattern	Image	Note
TS19: brain	Present		1G	Many labeled cells have engulfed dying cells and contain pyknotic nuclei.

Structure	Level	Pattern	Image	Note
TS19: liver	Present		1H	Expressed in cells showing stellate morphology and intimate association with smaller hematopoietic cells.

Structure	Level	Pattern	Image	Note
TS19: embryo	Present	Spotted	7A	Expressed in a speckled pattern throughout the embryo.
TS19: brain	Present	Spotted	7A	Expressed in a speckled pattern.
TS19: branchial arch	Present	Spotted	7A	Expressed in a speckled pattern.
TS19: heart	Present	Spotted	7A	Expressed in a speckled pattern.
TS19: limb	Present	Spotted	7A	Expressed in a speckled pattern.
TS19: liver	Present	Spotted	7A	Expressed in a speckled pattern.
TS19: body wall	Present	Regionally restricted	7A	Expressed along the dorsal midline and under the skin.

Structure	Level	Pattern	Image	Note
TS19: embryo	Present	Spotted	7B	Expressed in a speckled pattern throughout the embryo, same as in the wild-type.
TS19: brain	Present	Spotted	7B	Expressed in a speckled pattern, same as in the wild-type.
TS19: branchial arch	Present	Spotted	7B	Expressed in a speckled pattern, same as in the wild-type.
TS19: heart	Present	Spotted	7B	Expressed in a speckled pattern, same as in the wild-type.
TS19: limb	Present	Spotted	7B	Expressed in a speckled pattern, same as in the wild-type.
TS19: liver	Present	Spotted	7B	Expressed in a speckled pattern, same as in the wild-type.
TS19: body wall	Present	Regionally restricted	7B	Expressed along the dorsal midline and under the skin, same as in the wild-type.

Structure	Level	Pattern	Image	Note
TS19: liver	Present		7C

Structure	Level	Pattern	Image	Note
TS19: limb bud	Present		7D	Similar morphology and location to those in wild-type.

Structure	Level	Pattern	Image	Note
TS15: yolk sac	Present	Single cells		Expressed as isolated individual cells rather than clusters associated with blood islands.

Structure	Level	Pattern	Image	Note
TS16: heart	Present	Regionally restricted		Expressed a band overlying the developing heart.

Structure	Level	Pattern	Image	Note
TS19: eye	Present