Gata3 In situ reporter (knock in) Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:85304 Kaufman CK, et al., GATA-3: an unexpected regulator of cell lineage determination in skin. Genes Dev. 2003 Sep 1;17(17):2108-22
Assay type:	In situ reporter (knock in)
MGI Accession ID:	MGI:5760377
Gene symbol:	Gata3
Gene name:	GATA binding protein 3

Reporter:	lacZ
Antibody:	anti-B-galactosidase (rabbit)
Detection system:	Secondary antibody coupled to Texas Red

Results

Specimen 1C': embryonic day 13.0; Gata3^tm1Gsv/Gata3⁺ (more

)
Note: Post-fixed with 4% paraformaldehyde. Double labeled: red - Gata3 reporter; green - Krt5 (assay MGI:5760378).

Structure	Level	Pattern	Image	Note
TS21: epidermis	Absent		1C'

Specimen 1D': embryonic day 15.0; Gata3^tm1Gsv/Gata3⁺ (more

)
Note: Post-fixed with 4% paraformaldehyde. Double labeled: red - Gata3 reporter; green - Krt5 (assay MGI:5760378).

Structure	Level	Pattern	Image	Note
TS23: epidermis	Present	Regionally restricted	1D'	Expressed in the suprabasal layer. Expression were either absent or weak within the basal layer.

Specimen 1E': postnatal newborn; Gata3^tm1Gsv/Gata3⁺ (more

)
Note: Post-fixed with 4% paraformaldehyde. Double labeled: red - Gata3 reporter; green - Krt5 (assay MGI:5760378).

Structure	Level	Pattern	Image	Note
TS27: epidermis	Present	Regionally restricted	1E'	Expressed in both the suprabasal layer and the basal layer.
TS27: hair follicle	Present		1E'

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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