Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:83461
Patel TD, et al., Peripheral NT3 signaling is required for ETS protein expression and central patterning of proprioceptive sensory afferents. Neuron. 2003 May 8;38(3):403-16
|
|
Assay type:
|
Immunohistochemistry
|
|
MGI Accession ID:
|
MGI:5760025
|
|
Gene symbol:
|
Pvalb
|
|
Gene name:
|
parvalbumin
|
|
Antibody:
|
anti-parvalbumin (PV27) Rabbit
|
|
Detection system:
|
Secondary antibody coupled to Cy3
|
|
Assay notes:
|
Two antibodies were described, but the one used in this assay was not detailed. Anti-parvalbumin MGI:5760013 may have been used in this assay.
|
|
|
Results
|
Specimen
1B:
postnatal day 0
(more  )
Specimen
1B:
(close  )
|
Genetic Background:
|
involves: 129S4/SvJae * 129X1/SvJ * C57BL/6
|
|
Age:
|
postnatal day 0
|
|
Sex:
|
Not Specified
|
|
Type:
|
Not Specified
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS27: dorsal root ganglion |
Present |
Regionally restricted |
1B
|
Expression in large diameter proprioceptive neurons.
|
Specimen
1C:
(close )
|
Genetic Background:
|
involves: 129S4/SvJae * C57BL/6
|
|
Mutant Allele(s):
|
Ntf3tm1Jae/Ntf3tm1Jae
|
|
Age:
|
postnatal day 0
|
|
Sex:
|
Not Specified
|
|
Type:
|
Not Specified
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS27: dorsal root ganglion |
Trace |
|
1C
|
Authors state that the number of expressing neurons was dramatically reduced.
|
Specimen
1D:
(close )
|
Genetic Background:
|
involves: 129S4/SvJae * 129X1/SvJ * C57BL/6
|
|
Mutant Allele(s):
|
Baxtm1Sjk/Baxtm1Sjk
Ntf3tm1Jae/Ntf3tm1Jae
|
|
Age:
|
postnatal day 0
|
|
Sex:
|
Not Specified
|
|
Type:
|
Not Specified
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS27: dorsal root ganglion |
Present |
Regionally restricted |
1D
|
Authors report expression in numerous neurons. These neurons are considerably smaller than expressing neurons in wild-type dorsal root ganglia.
|
Specimen
5A:
embryonic day 15.0
(more )
Specimen
5A:
(close )
|
Genetic Background:
|
involves: 129S4/SvJae * 129X1/SvJ * C57BL/6
|
|
Age:
|
embryonic day 15.0
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
Specimen
5B:
(close )
|
Genetic Background:
|
involves: 129S4/SvJae * C57BL/6
|
|
Mutant Allele(s):
|
Ntf3tm1Jae/Ntf3tm1Jae
|
|
Age:
|
embryonic day 15.0
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
Specimen
5C:
(close )
|
Genetic Background:
|
involves: 129S4/SvJae * 129X1/SvJ * C57BL/6
|
|
Mutant Allele(s):
|
Baxtm1Sjk/Baxtm1Sjk
Ntf3tm1Jae/Ntf3tm1Jae
|
|
Age:
|
embryonic day 15.0
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS23: dorsal root ganglion |
Weak |
Regionally restricted |
5C
|
Expression in neurons. Authors report the cross-sectional area, of expressing neurons was equivalent to that seen in wild-type, but that the intensity of expression appeared to be fainter.
|
|
Analysis Tools
