Gene Expression Data
Assay Details
Assay
|
Reference:
|
J:63083
Clarris HJ, et al., Dynamic spatiotemporal expression patterns of neurocan and phosphacan indicate diverse roles in the developing and adult mouse olfactory system. J Comp Neurol. 2000 Jul 17;423(1):99-111
|
Assay type:
|
Immunohistochemistry
|
MGI Accession ID:
|
MGI:5758932
|
Gene symbol:
|
Ncan
|
Gene name:
|
neurocan
|
Antibody:
|
anti-neurocan
|
Detection system:
|
Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
|
|
Results
|
Specimen
E11.5 not shown:
embryonic day 11.5
(more )
Specimen
E11.5 not shown:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 11.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: olfactory epithelium |
Present |
Regionally restricted |
|
In the olfactory neuroepithelium, expression barely detectable in perikarya of primary sensory neurons, but clearly localized to axons.
|
Specimen
1A:
embryonic day 12.5
(more )
Specimen
1A:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: olfactory epithelium |
Present |
Regionally restricted |
1A
|
Expression localized to perikarya of a subpopulation of primary olfactory neurons in the olfactory epithelium.
|
TS20: olfactory nerve |
Present |
Regionally restricted |
1A
|
Expression in olfactory axons between olfactory epithelium and migratory mass.
|
TS20: olfactory migratory mass |
Present |
|
1A
|
Expression within the migratory mass, clearly distinct from the telencephalon.
|
Specimen
2A:
embryonic day 20.5
(more )
Specimen
2A:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 20.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS26: olfactory epithelium |
Present |
Regionally restricted |
2A
|
Expression in olfactory nerve fascicles in the lamina propria. Weak expression in N-CAM-expressing neuronal perikarya, deep to OMP-expressing perikarya.
|
Specimen
2B:
postnatal day 3
(more )
Specimen
2B:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
postnatal day 3
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS27: olfactory epithelium |
Present |
Regionally restricted |
2B
|
Expression in a markedly increased number of perikarya within a distinct layer occupying the middle one-third of the neuroepithelium.
|
Specimen
2C:
postnatal adult
(more )
Specimen
2C:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
postnatal adult
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: olfactory epithelium |
Present |
Widespread |
2C
|
Expression throughout the neuroepithelium occupied by primary olfactory neurons.
|
Specimen
4A:
embryonic day 20.5
(more )
Specimen
4A:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 20.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Specimen
4C:
embryonic day 20.5
(more )
Specimen
4C:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 20.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Specimen
4E:
postnatal day 3
(more )
Specimen
4E:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
postnatal day 3
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS27: main olfactory bulb external plexiform layer |
Present |
Regionally restricted |
4E
|
Expression in the neuropil of the outer external plexiform layer. Expression in external plexiform layer is consistent with localization in apical dendrites of mitral and granule cells. No expression in perikarya of these neurons.
|
TS27: main olfactory bulb glomerular layer |
Present |
Diffuse |
4E
|
Authors state that diffuse expression in outer layers of the olfactory bulb made it difficult to discern individual glomeruli.
|
Specimen
4G:
postnatal adult
(more )
Specimen
4G:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
postnatal adult
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Specimen
E13.5 not shown:
embryonic day 13.5
(more )
Specimen
E13.5 not shown:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 13.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: vomeronasal organ |
Present |
Regionally restricted |
|
Expression within perikarya of a subset of neurons.
|
Specimen
8A:
embryonic day 20.5
(more )
Specimen
8A:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 20.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS26: vomeronasal organ |
Present |
Regionally restricted |
8A
|
Expression within perikarya of a subset of neurons. Strong expression in fascicles of axons in submucosa.
|
Specimen
8B:
postnatal day 3
(more )
Specimen
8B:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
postnatal day 3
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS27: vomeronasal organ |
Present |
Regionally restricted |
8B
|
Expression within perikarya of a subset of neurons. Strong expression in fascicles of axons in submucosa.
|
Specimen
8C:
postnatal adult
(more )
Specimen
8C:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
postnatal adult
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: vomeronasal organ |
Present |
Regionally restricted |
8C
|
Decreased expression in cell bodies of neurons. Expression continues to be present within fascicles of axons.
|
Specimen
8G:
postnatal day 14
(more )
Specimen
8G:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
postnatal day 14
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
|