Probe:	p4.6
Probe preparation:	Antisense labelled with S35 RNA
Assay notes:	Authors described two probes that gave identical results. Either p3.6 (MGI:5447309) or p4.6 (MGI:5756056) could have been used in this assay. Assay MGI:5756055 has data with images.

Structure	Level	Pattern	Image	Note
TS17: embryo	Absent

Structure	Level	Pattern	Image	Note
TS22: lower jaw incisor mesenchyme	Strong
TS22: upper jaw molar mesenchyme	Strong
TS22: shoulder joint primordium	Present	Regionally restricted		Expression is at the articular surfaces of the shoulder joint.
TS22: humerus cartilage condensation	Present	Regionally restricted		Expression is at the perichondrial cartilage and in interstitial cells.
TS22: scapula cartilage condensation	Present	Regionally restricted		Expression is at the perichondrial cartilage and in interstitial cells.
TS22: jaw mesenchyme	Present	Regionally restricted		Expression is at the opposing cartilagenous elements and in cells at either side of the midline of the jaw.
TS22: cranium	Present	Regionally restricted		Expression is at and between the articular surfaces of the developing head skeleton.
TS22: nasal septum cartilage	Present	Regionally restricted		Expression is at the proximal end of the medial nasal cartilage.
TS22: rib cartilage condensation	Present	Regionally restricted		Punctate expression in the perichondrium.
TS22: jaw skeleton	Present	Regionally restricted		Expression is in alveolar bone forming around incisor tooth germ.
TS22: forelimb musculature	Present	Regionally restricted		Expression is higher where tendons are forming.
TS22: vibrissa follicle	Present	Regionally restricted		Expression is in cells of the inner root sheath.
TS22: nasal mesenchyme	Present	Regionally restricted		Expression is in the mesenchymal tissue surrounding the epithelium of the nasal glands, absent in non-gland associated mesenchyme.
TS22: salivary gland mesenchyme	Present	Regionally restricted		Expression is in the condensed mesenchyme surrounding the epithelium, absent in the salivary duct.
TS22: tongue muscle	Present	Patchy		Expression is in the central muscle and in the connective tissue sheath and developing tendons.
TS22: brain	Absent
TS22: hair follicle dermal papilla	Absent
TS22: lower jaw incisor epithelium	Absent
TS22: upper jaw molar epithelium	Absent

Structure	Level	Pattern	Image	Note
TS25: forelimb joint	Present	Regionally restricted		Expression is in epiphyseal cartilage at the articular surfaces of the developing limbs and in the interstitial cells that lie in between cartilaginous elements especially in the distal regions.
TS25: forelimb skeleton	Present	Regionally restricted		Expression is in epiphyseal cartilage at the articular surfaces of the developing limbs and in the interstitial cells that lie in between cartilaginous elements especially in the distal regions.
TS25: jaw skeleton	Present	Regionally restricted		Expression is in alveolar bone forming around incisor tooth germ.
TS25: vibrissa follicle	Present	Regionally restricted		Expression is in the inner root sheath, absent in the central region of the hair shaft.
TS25: tooth	Weak	Regionally restricted		Expression is in the pulp.