Probe:	p3.6
Probe preparation:	Antisense labelled with S35 RNA
Assay notes:	Authors described two probes that gave identical results. Either p3.6 (MGI:5447309) or p4.6 (MGI:5756056) could have been used in this assay.

Structure	Level	Pattern	Image	Note
TS17: embryo	Absent

Structure	Level	Pattern	Image	Note
TS22: shoulder joint primordium	Present	Regionally restricted	2A/B	Expression is at the articular surfaces of the shoulder joint.
TS22: humerus cartilage condensation	Present	Regionally restricted	2A/B	Expression is at the perichondrial cartilage and in interstitial cells.
TS22: scapula cartilage condensation	Present	Regionally restricted	2A/B	Expression is at the perichondrial cartilage and in interstitial cells.

Structure	Level	Pattern	Image	Note
TS25: forelimb joint	Present	Regionally restricted	2C/D , 2E/F	Expression is in epiphyseal cartilage at the articular surfaces of the developing limbs and in the interstitial cells that lie in between cartilaginous elements especially in the distal regions.
TS25: forelimb skeleton	Present	Regionally restricted	2C/D , 2E/F	Expression is in epiphyseal cartilage at the articular surfaces of the developing limbs and in the interstitial cells that lie in between cartilaginous elements especially in the distal regions.

Structure	Level	Pattern	Image	Note
TS22: jaw mesenchyme	Present	Regionally restricted	2G/H	Expression is at the opposing cartilagenous elements and in cells at either side of the midline of the jaw.
TS22: cranium	Present	Regionally restricted	2G/H	Expression is at and between the articular surfaces of the developing head skeleton.
TS22: brain	Absent		2G/H

Structure	Level	Pattern	Image	Note
TS22: nasal septum cartilage	Present	Regionally restricted	2I/J	Expression is at the proximal end of the medial nasal cartilage.
TS22: olfactory epithelium	Absent		2I/J

Structure	Level	Pattern	Image	Note
TS22: rib cartilage condensation	Present	Regionally restricted	2K/L	Punctate expression in the perichondrium.

Structure	Level	Pattern	Image	Note
TS22: humerus cartilage condensation	Strong	Regionally restricted	3A/B	Expression is at periosteal regions.

Structure	Level	Pattern	Image	Note
TS25: jaw skeleton	Present	Regionally restricted	3C/D	Expression is in alveolar bone forming around incisor tooth germ.

Structure	Level	Pattern	Image	Note
TS22: lower jaw incisor mesenchyme	Strong		3E/F
TS22: upper jaw molar mesenchyme	Strong		3E/F
TS22: lower jaw incisor epithelium	Absent		3E/F
TS22: upper jaw molar epithelium	Absent		3E/F

Structure	Level	Pattern	Image	Note
TS22: forelimb musculature	Present	Patchy	4A/B	Expression is higher where tendons are forming.
TS22: humerus cartilage condensation	Present	Regionally restricted	4A/B	Expression is in interstitial cells at the surface of the bone.
TS22: scapula cartilage condensation	Present	Regionally restricted	4A/B	Expression is in interstitial cells at the surface of the bone.

Structure	Level	Pattern	Image	Note
TS22: tongue muscle	Present	Patchy	4C/D	Expression is in the central muscle and in the connective tissue sheath and developing tendons.

Structure	Level	Pattern	Image	Note
TS22: vibrissa follicle	Present	Regionally restricted	5A/B	Expression is in cells of the inner root sheath.
TS22: hair follicle dermal papilla	Absent		5A/B

Structure	Level	Pattern	Image	Note
TS25: vibrissa follicle	Present	Regionally restricted	5C/D	Expression is in the inner root sheath, absent in the central region of the hair shaft.

Structure	Level	Pattern	Image	Note
TS22: nasal mesenchyme	Present	Regionally restricted	6A/B	Expression is in the mesenchymal tissue surrounding the epithelium of the nasal glands, absent in non-gland associated mesenchyme and in nasal cartilage.
TS22: olfactory epithelium	Absent		6A/B

Structure	Level	Pattern	Image	Note
TS22: salivary gland mesenchyme	Present	Regionally restricted	6C/D	Expression is in the condensed mesenchyme surrounding the epithelium, absent in the salivary duct.

Structure	Level	Pattern	Image	Note
TS25: tooth	Weak	Patchy		Expression is in the pulp.