Summary | Mutation origin | Mutation description | Expression | Phenotypes | Find Mice (IMSR) | References

Symbol:	Ntn1tm1.2Tek
Name:	netrin 1; targeted mutation 1.2, Timothy E Kennedy
MGI ID:	MGI:5752968
Synonyms:	Netrin-1-
Gene:	Ntn1  Location: Chr11:68100190-68277652 bp, - strand  Genetic Position: Chr11, 41.45 cM, cytoband B3
Alliance:	Ntn1tm1.2Tek page

Germline Transmission:	Earliest citation of germline transmission: J:227232
Parent Cell Line:	R1 (ES Cell)
Strain of Origin:	(129X1/SvJ x 129S1/Sv)F1-Kitl+

Allele Type:    Targeted (Null/knockout) Mutation:    Intragenic deletion   Mutation details: The targeting vector contains a FRT-flanked Neo selection cassette and a loxP site was inserted downstream of the first protein coding exon of the targeted gene, and another loxP site was inserted upstream of the first protein coding exon. Flp-mediated recombination removed the FRT-flanked neo cassette. Cre-mediated recombination removed the first protein coding exon. Western blot and immunohistochemical analysis of spinal cord confirmed the absence of protein expression. (J:227232)

View phenotypes and curated references for all genotypes (concatenated display).

In Structures Affected by this Mutation:

3 anatomical structure(s)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Ntn1 Mutation:	106 strains or lines available

Original:	J:227232 Bin JM, et al., Complete Loss of Netrin-1 Results in Embryonic Lethality and Severe Axon Guidance Defects without Increased Neural Cell Death. Cell Rep. 2015 Aug 18;12(7):1099-106
All:	2 reference(s)