Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:41401
Grindley JC, et al., Disruption of PAX6 function in mice homozygous for the Pax6Sey-1Neu mutation produces abnormalities in the early development and regionalization of the diencephalon. Mech Dev. 1997 Jun;64(1-2):111-26
|
|
Assay type:
|
RNA in situ
|
|
MGI Accession ID:
|
MGI:5752922
|
|
Gene symbol:
|
Sim2
|
|
Gene name:
|
single-minded family bHLH transcription factor 2
|
|
Probe:
|
mSim2 probe
|
|
Probe preparation:
|
Antisense labelled with S35 RNA
|
|
|
Results
|
Specimen
4A:
embryonic day 12.5
(more  )
Specimen
4A:
(close  )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 12.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS20: diencephalon |
Present |
Regionally restricted |
4A
|
Expression in narrow bands along the zona limitans.
|
Specimen
4B:
(close )
|
Genetic Background:
|
involves: 102 * C3H
|
|
Mutant Allele(s):
|
Pax6Sey-Neu/Pax6Sey-Neu
|
|
Age:
|
embryonic day 12.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS20: diencephalon |
Present |
Regionally restricted |
4B
|
Enlargement of zona limitans expression domain in antero-posterior direction compared with control.
|
Specimen
4C:
embryonic day 12.5
(more )
Specimen
4C:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 12.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS20: diencephalon |
Present |
Regionally restricted |
4C
|
Expression marks position of zona limitans.
|
Specimen
4E:
(close )
|
Genetic Background:
|
involves: 102 * C3H
|
|
Mutant Allele(s):
|
Pax6Sey-Neu/Pax6Sey-Neu
|
|
Age:
|
embryonic day 12.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS20: diencephalon |
Present |
Regionally restricted |
4E
|
Blurring of posterior prosomere 2 boundary, but not anterior prosomere 3 boundary.
|
Specimen
5I:
embryonic day 12.5
(more )
Specimen
5I:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 12.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS20: diencephalon |
Present |
Regionally restricted |
5I
|
Major anterior boundary of expression in hypothalamus.
|
Specimen
5J:
(close )
|
Genetic Background:
|
involves: 102 * C3H
|
|
Mutant Allele(s):
|
Pax6Sey-Neu/Pax6Sey-Neu
|
|
Age:
|
embryonic day 12.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
|
Analysis Tools