Gene Expression Data
Assay Details
Assay
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Reference:
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J:148786
Barber M, et al., The role of Robo3 in the development of cortical interneurons. Cereb Cortex. 2009 Jul;19 Suppl 1:i22-31
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:5752757
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Gene symbol:
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Calb1
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Gene name:
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calbindin 1
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Results
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Specimen
3A:
embryonic day 18.5
(more )
Specimen
3A:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Structure |
Level |
Pattern |
Image |
Note |
TS26: cerebral cortex |
Present |
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3A
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Expressed in the cortical interneurons.
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Specimen
3B:
(close )
Genetic Background:
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Not Specified
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Mutant Allele(s):
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Robo3tm1Matl/Robo3tm1Matl
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS26: cerebral cortex |
Present |
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3B
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Expressed in the cortical interneurons.
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Specimen
S2D:
embryonic day 13.5; Robo3tm1Matl/Robo3 +
(more )
Specimen
S2D:
(close )
Genetic Background:
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Not Specified
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Mutant Allele(s):
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Robo3tm1Matl/Robo3+
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: cerebral cortex |
Present |
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S2D
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Expressed in the cortical interneurons.
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Specimen
S2E:
(close )
Genetic Background:
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Not Specified
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Mutant Allele(s):
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Robo3tm1Matl/Robo3tm1Matl
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Age:
|
embryonic day 13.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: cerebral cortex |
Present |
|
S2E
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Expressed in the cortical interneurons.
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Specimen
not shown:
embryonic day 13.5; Robo3tm1Matl/Robo3 +
(more )
Note: Data was summarized in Fig. 3C, 3D.
Specimen
not shown:
(close )
Genetic Background:
|
Not Specified
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Mutant Allele(s):
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Robo3tm1Matl/Robo3+
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
|
Cryosection
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Note:
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Data was summarized in Fig. 3C, 3D.
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Specimen
not shown:
(close )
Genetic Background:
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Not Specified
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Mutant Allele(s):
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Robo3tm1Matl/Robo3tm1Matl
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Age:
|
embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
|
Embedding:
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Cryosection
|
Note:
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Data was summarized in Fig. 3C, 3D.
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: cerebral cortex |
Present |
|
|
Expressed in the cortical interneurons. No significant changes in the total number of positive cells within rostral-middle or caudal levels, compared with the heterozygous control.
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TS21: cerebral cortex marginal layer |
Present |
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Expressed in the cortical interneurons. There was a significant reduction in the number of positive cells compared with heterozygote littermates in rostral-middle levels and caudal levels of the cortex.
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Specimen
not shown:
embryonic day 18.5; Robo3tm1Matl/Robo3 +
(more )
Note: Data was summarized in Fig. 3E, 3F.
Specimen
not shown:
(close )
Genetic Background:
|
Not Specified
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Mutant Allele(s):
|
Robo3tm1Matl/Robo3+
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Age:
|
embryonic day 18.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
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Data was summarized in Fig. 3E, 3F.
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Specimen
not shown:
(close )
Genetic Background:
|
Not Specified
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Mutant Allele(s):
|
Robo3tm1Matl/Robo3tm1Matl
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Age:
|
embryonic day 18.5
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Sex:
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Not Specified
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Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Data was summarized in Fig. 3E, 3F.
|
Structure |
Level |
Pattern |
Image |
Note |
TS26: cerebral cortex |
Present |
|
|
Expressed in the cortical interneurons. There was no differences in the number of positive cells in rostral-middle levels. A small but just significant decrease (11%) was observed within the caudal relative to heterozygote littermates.
|
Specimen
not shown:
(close )
Specimen
not shown:
(close )
Structure |
Level |
Pattern |
Image |
Note |
TS21: cerebral cortex |
Present |
|
|
Expressed in the cortical interneurons. There was a decrease in the total number of labeled cells relative to an animal which was heterozygote for both Robo1 and Robo2 receptors and fully deficient for the Robo3 receptor.
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Specimen
not shown:
embryonic day 18.5; Robo3tm1Matl/Robo3 +
(more )
Specimen
not shown:
(close )
Genetic Background:
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involves: 129S/SvEv * C57BL/6
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Mutant Allele(s):
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Robo3tm1Matl/Robo3+
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Specimen
not shown:
(close )
Structure |
Level |
Pattern |
Image |
Note |
TS26: cerebral cortex |
Present |
|
|
Expressed in the cortical interneurons. Approximately 38%, 30% decrease in the number of positive cells within rostral-middle and caudal regions respectively, compared with a single Robo3 heterozygote littermate.
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