Gene Expression Data
Assay Details
Assay
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Reference:
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J:148786
Barber M, et al., The role of Robo3 in the development of cortical interneurons. Cereb Cortex. 2009 Jul;19 Suppl 1:i22-31
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:5752750
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Gene symbol:
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Reln
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Gene name:
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reelin
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Antibody:
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G10
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Detection system:
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Secondary antibody coupled to Alexa Fluor 568
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Results
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Specimen
2A/A':
embryonic day 13.5
(more )
Specimen
2A/A':
(close )
Genetic Background:
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C57BL/6J
|
Age:
|
embryonic day 13.5
|
Sex:
|
Not Specified
|
Type:
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section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: cerebral cortex |
Strong |
Regionally restricted |
2A
,
2A'
|
Expressed in a single layer cells (Cajal--Retzius cells) outside of the preplate/marginal zone.
|
Specimen
2B/B':
embryonic day 13.5
(more )
Specimen
2B/B':
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
embryonic day 13.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: cerebral cortex |
Strong |
Regionally restricted |
2B'
,
2B
|
Expressed in a single layer cells (Cajal--Retzius cells) outside of the preplate/marginal zone.
|
Specimen
2C/C':
embryonic day 13.5
(more )
Specimen
2C/C':
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
embryonic day 13.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: cerebral cortex |
Strong |
Regionally restricted |
2C
,
2C'
|
Expressed in a single layer cells (Cajal--Retzius cells) outside of the preplate/marginal zone.
|
Specimen
not shown:
embryonic day 13.5; Robo3tm1Matl/Robo3 +
(more )
Specimen
not shown:
(close )
Genetic Background:
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Not Specified
|
Mutant Allele(s):
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Robo3tm1Matl/Robo3+
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Age:
|
embryonic day 13.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: cortical preplate |
Present |
|
|
Expressed in the preplate of the developing neocortex and hippocampus.
|
Specimen
not shown:
(close )
Genetic Background:
|
Not Specified
|
Mutant Allele(s):
|
Robo3tm1Matl/Robo3tm1Matl
|
Age:
|
embryonic day 13.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: cortical preplate |
Present |
Regionally restricted |
|
Expressed in the preplate of the developing neocortex and hippocampus. No significant changes in the total number of positive cells, compared with the heterozygous control.
|
Specimen
not shown:
embryonic day 18.5
(more )
Specimen
not shown:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 18.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Specimen
not shown:
(close )
Genetic Background:
|
Not Specified
|
Mutant Allele(s):
|
Robo3tm1Matl/Robo3tm1Matl
|
Age:
|
embryonic day 18.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS26: cerebral cortex marginal zone |
Present |
|
|
No significant changes in the total number of positive cells, compared with the wild-type littermates.
|
Specimen
not shown:
embryonic day 12.5
(more )
Specimen
not shown:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: cortical preplate |
Present |
|
|
Expressed in the preplate of the developing neocortex and hippocampus.
|
Specimen
not shown:
(close )
Genetic Background:
|
involves: 129S/SvEv
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Mutant Allele(s):
|
Robo1tm1Wian/Robo1tm1Wian
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Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: cortical preplate |
Present |
|
|
Expressed in the preplate of the developing neocortex and hippocampus. No significant changes in the total number of positive cells, compared with the wild-type littermates.
|
Specimen
not shown:
embryonic day 12.5
(more )
Specimen
not shown:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: cortical preplate |
Present |
|
|
Expressed in the preplate of the developing neocortex and hippocampus.
|
Specimen
not shown:
(close )
Genetic Background:
|
involves: 129
|
Mutant Allele(s):
|
Robo2tm1.1Rilm/Robo2tm1.1Rilm
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: cortical preplate |
Present |
Regionally restricted |
|
Expressed in the preplate of the developing neocortex and hippocampus. No significant changes in the total number of positive cells in the neocortex, but a significant increase (38%) in the hippocampal region, compared with the wild-type littermates.
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