Probe:	Sfrp2 cDNA3
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS17: forebrain	Present	Non-Uniform	1A	Weak expression was found throughout the forebrain. Higher expression in the diencephalon had a posterior boundary in zona limitans intrathalamica, a rostral p4/p5 limit, and dorsally in the eminentia thalami as it entered the telencephalon.
TS17: diencephalon	Present	Regionally restricted	1A	There was a sharply demarcated region of higher expression in a restricted domain in the diencephalon.
TS17: telencephalon	Present	Regionally restricted	1A	Expression was detected in the caudal telencephalon.

Structure	Level	Pattern	Image	Note
TS22: forebrain	Present	Non-Uniform	1B	The E10.5 pattern of expression was maintained at E14.5.
TS22: diencephalon	Present	Regionally restricted	1B	Expression was detected in the eminentia thalami.
TS22: telencephalon	Present	Regionally restricted	1B	Expression was detected in the ventral pallium.

Structure	Level	Pattern	Image	Note
TS22: forebrain	Present	Non-Uniform	1C	Expression in p5 was more prominent than at E10.5. Expression was detected in the developing pallial-sub-pallial boundary.
TS22: diencephalon	Present		1C
TS22: caudal ganglionic eminence	Present	Regionally restricted	1C	Expression was detected in a small domain flanking the caudal ganglionic eminence.

Structure	Level	Pattern	Image	Note
TS22: caudal ganglionic eminence	Present		1D

Structure	Level	Pattern	Image	Note
TS24: amygdala	Present	Scattered	1E
TS24: neocortex	Present	Scattered	1E
TS24: diencephalon	Present		1E
TS24: caudal ganglionic eminence	Present		1E

Structure	Level	Pattern	Image	Note
TS27: diencephalon	Absent

Structure	Level	Pattern	Image	Note
TS28: amygdala	Present	Scattered	1F
TS28: neocortex	Present	Scattered	1F
TS28: piriform cortex	Present	Scattered	1F
TS28: nucleus of diagonal band	Present	Regionally restricted	1F	Expression was detected in the vertical limb of the diagonal band.

Structure	Level	Pattern	Image	Note
TS21: telencephalon	Present	Regionally restricted	2B , 2D	Expression was detected in the pallial side of the pallial-sub-pallial boundary, in the ventricular zone of the ventral pallium. Expression overlaps with Pax6, and abuts the Dlx2 expression.

Structure	Level	Pattern	Image	Note
TS21: diencephalon	Present		3A
TS21: telencephalon	Present	Regionally restricted	3A	Expression was detected in the ventral pallium of the pallial-sub-pallial boundary.

Structure	Level	Pattern	Image	Note
TS21: diencephalon	Absent		3B
TS21: telencephalon	Absent		3B	Expression was undetectable in the ventral pallium.

Structure	Level	Pattern	Image	Note
TS21: diencephalon	Present			Expression in the mutant was unaffected.
TS21: telencephalon	Present	Regionally restricted		Expression in the mutant ventral pallium was unaffected.

Structure	Level	Pattern	Image	Note
TS22: diencephalon	Present			Expression in the mutant was unaffected.
TS22: telencephalon	Present	Regionally restricted		Expression in the mutant ventral pallium was unaffected.

Structure	Level	Pattern	Image	Note
TS24: diencephalon	Present			Expression in the mutant was unaffected.
TS24: telencephalon	Present	Regionally restricted		Expression in the mutant ventral pallium was unaffected.

Structure	Level	Pattern	Image	Note
TS17: future spinal cord	Present	Regionally restricted	4A	Expression was detected in the intermediate and ventral zones of the spinal cord.
TS17: dorsal root ganglion	Absent		4A

Structure	Level	Pattern	Image	Note
TS17: future spinal cord	Weak		4B	Expression was greatly reduced in the mutant.
TS17: dorsal root ganglion	Absent		4B