Gene Expression Data
Assay Details
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Assay
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Reference:
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J:116230
Huber K, et al., Cholinergic differentiation occurs early in mouse sympathetic neurons and requires Phox2b. Gene Expr. 2006;13(2):133-9
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:5751439
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Gene symbol:
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Slc18a3
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Gene name:
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solute carrier family 18 (vesicular monoamine), member 3
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Probe:
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VAChT cDNA
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
1B:
embryonic day 11.5
(more  )
Specimen
1B:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
1E:
embryonic day 11.5
(more )
Specimen
1E:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
1H:
embryonic day 11.5
(more )
Specimen
1H:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: sympathetic ganglion |
Present |
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1H
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Expression is in the lower thoracic to lumbal adrenal and sympathetic ganglion anlagen.
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Specimen
2B:
embryonic day 11.5
(more )
Specimen
2B:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: sympathetic ganglion |
Strong |
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2B
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Expression is in most cells of the cervical sympathetic ganglion.
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Specimen
2D:
embryonic day 11.5
(more )
Specimen
2D:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: sympathetic nervous system |
Present |
Regionally restricted |
2D
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Expression is found mostly dorsolateral to the aorta in presumptive ganglionic sites, less frequent at the site of the adrenal anlage.
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Specimen
3B:
embryonic day 13.5
(more )
Specimen
3B:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: adrenal gland |
Present |
Single cells |
3B
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Expression is detected in only a few cells of adrenal tissue.
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| TS21: sympathetic ganglion |
Present |
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3B
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Expression is in ganglion cell clusters dorsal and ventral to the adrenal anlagen.
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Specimen
3E:
embryonic day 13.5
(more )
Specimen
3E:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: adrenal gland |
Present |
Single cells |
3E
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Expression is detected in only a few cells of adrenal tissue.
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| TS21: sympathetic ganglion |
Present |
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3E
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Expression is in ganglion cell clusters dorsal and ventral to the adrenal anlagen.
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Specimen
4C:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Phox2btm1Jbr/Phox2btm1Jbr
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
4F:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Phox2btm1Jbr/Phox2btm1Jbr
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: sympathetic ganglion |
Absent |
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4F
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Shown is the lower thoracic to lumbal adrenal and sympathetic ganglion anlagen.
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Specimen
not shown:
embryonic day 10.5
(more )
Specimen
not shown:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
not shown +/-:
embryonic day 11.5; Phox2btm1Jbr/Phox2b +
(more )
Specimen
not shown +/-:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Phox2btm1Jbr/Phox2b+
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Analysis Tools