Gene Expression Data
Assay Details
Assay
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Reference:
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J:202865
Yang T, et al., Prickle1 stunts limb growth through alteration of cell polarity and gene expression. Dev Dyn. 2013 Nov;242(11):1293-306
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:5751414
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Gene symbol:
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Vangl2
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Gene name:
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VANGL planar cell polarity 2
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Probe:
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Vangl2 cDNA3
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
5I:
embryonic day 12.5
(more )
Specimen
5I:
(close )
Genetic Background:
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involves: C57BL/6
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Structure |
Level |
Pattern |
Image |
Note |
TS20: forelimb |
Present |
Widespread |
5I
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Expression in the whole limb bud.
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TS20: forelimb digit |
Strong |
Regionally restricted |
5I
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Expression concentrated in the distal tips.
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Specimen
5J:
(close )
Genetic Background:
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C57BL/6-Prickle1m2Ingm
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Mutant Allele(s):
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Prickle1m2Ingm/Prickle1m2Ingm
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Structure |
Level |
Pattern |
Image |
Note |
TS20: hand |
Present |
Regionally restricted |
5J
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Reduced expression in posterior limb region in mutants compared with wild type.
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