Gene Expression Data
Assay Details
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Assay
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Reference:
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J:202865
Yang T, et al., Prickle1 stunts limb growth through alteration of cell polarity and gene expression. Dev Dyn. 2013 Nov;242(11):1293-306
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:5751413
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Gene symbol:
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Prickle1
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Gene name:
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prickle planar cell polarity protein 1
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Probe:
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Prickle1 cDNA2
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
5G:
embryonic day 12.5
(more  )
Specimen
5G:
(close  )
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Genetic Background:
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involves: C57BL/6
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
5H:
(close )
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Genetic Background:
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C57BL/6-Prickle1m2Ingm
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Mutant Allele(s):
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Prickle1m2Ingm/Prickle1m2Ingm
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: hand |
Weak |
Regionally restricted |
5H
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Lower general expression in the mutant than in wild type.
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| TS20: forelimb digit |
Present |
Regionally restricted |
5H
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No obvious segmentation of expression in digits.
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Specimen
6K:
embryonic day 11.5
(more )
Specimen
6K:
(close )
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Genetic Background:
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involves: C57BL/6
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: handplate |
Present |
Regionally restricted |
6K
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Authors report variable expression in the limb bud. Authors state that expression might be in developing skeleton condensates (future digits).
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Specimen
6L:
(close )
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Genetic Background:
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C57BL/6-Prickle1m2Ingm
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Mutant Allele(s):
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Prickle1m2Ingm/Prickle1m2Ingm
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: handplate |
Trace |
Regionally restricted |
6L
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Expression barely detectable, especially in the anterior limb bud.
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Specimen
not shown:
embryonic day 10.5
(more )
Specimen
not shown:
(close )
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Genetic Background:
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involves: C57BL/6
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: forelimb bud |
Absent |
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Authors report no detectable patterned expression at E10.5
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Specimen
9C inset:
embryonic day 15.5
(more )
Specimen
9C inset:
(close )
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Genetic Background:
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involves: C57BL/6
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: radius |
Present |
Regionally restricted |
9C inset
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Expression at ends of radius and ulna.
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| TS23: ulna |
Present |
Regionally restricted |
9C inset
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Expression at ends of radius and ulna.
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Specimen
9D inset:
(close )
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Genetic Background:
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C57BL/6-Prickle1m2Ingm
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Mutant Allele(s):
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Prickle1m2Ingm/Prickle1m2Ingm
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: radius |
Present |
Regionally restricted |
9D inset
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Expression at ends of radius and ulna.
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| TS23: ulna |
Present |
Regionally restricted |
9D inset
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Expression at ends of radius and ulna.
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