Marf1 RT-PCR Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:191238 Su YQ, et al., Meiosis arrest female 1 (MARF1) has nuage-like function in mammalian oocytes. Proc Natl Acad Sci U S A. 2012 Nov 13;109(46):18653-60
Assay type:	RT-PCR
MGI Accession ID:	MGI:5749208
Gene symbol:	Marf1
Gene name:	meiosis regulator and mRNA stability 1

Probe:	Marf1-pA, Marf1-pB
Assay notes:	Real-time RT-PCR assay. Relative expression is shown as a graph normalized to Rpl19.

Results

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	Size Not Specified	Amount	Genetic Background	Mutant Allele(s)	Sex
GV-FGO	P	TS28: primary oocyte	Present	Not Specified	Not Specified		Female
M II	P	TS28: secondary oocyte	Present (a)	Not Specified	Not Specified		Female
1-cell	E0.5 (b)	TS1: 1-cell stage conceptus	Present (a)	Not Specified	involves: C57BL/6J * SJL/J		Not Specified
2-cell	E1.0 (b)	TS2: 2-cell stage conceptus	Weak (c)	Not Specified	involves: C57BL/6J * SJL/J		Not Specified
4-cell	E2.0 (b)	TS3: 4-cell stage conceptus	Weak (c)	Not Specified	involves: C57BL/6J * SJL/J		Not Specified
8-cell	E2.5 (b)	TS3: 8-cell stage conceptus	Weak (c)	Not Specified	involves: C57BL/6J * SJL/J		Not Specified
Morula	E3.0 (b)	TS3: morula-stage conceptus	Weak (c)	Not Specified	involves: C57BL/6J * SJL/J		Not Specified
Blastocyst	E3.5 (b)	TS4: blastocyst-stage conceptus	Weak (c)	Not Specified	involves: C57BL/6J * SJL/J		Not Specified

Notes:
(a) Expression was reduced to approximately half the levels seen at GV-FGO.
(b) Age assigned by curator based on morphological criteria supplied by authors.
(c) Expression was reduced to approximately 5% of the levels seen at GV-FGO.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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