Gpx2 RT-PCR Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:186299 Baek IJ, et al., Differential expression of gastrointestinal glutathione peroxidase (GI-GPx) gene during mouse organogenesis. Anat Histol Embryol. 2011 Jun;40(3):210-8
Assay type:	RT-PCR
MGI Accession ID:	MGI:5708675
Gene symbol:	Gpx2
Gene name:	glutathione peroxidase 2

Probe:	GI-GPx-pA, GI-GPx-pB
Probe preparation:	labelled with FAM
Assay notes:	This assay is a quantitative RT-PCR, Gapdh was used as the standard.

Results

Image: 1

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	Size Not Specified (a)	Amount	Genetic Background	Mutant Allele(s)	Sex	Note
E7.5 left	E7.5	TS11: embryo	Present	5 µg; total RNA	ICR		Not Specified
E7.5 right	E7.5	TS11: extraembryonic component	Present	5 µg; total RNA	ICR		Not Specified	Extraembryonic tissues included ectoplacental cone (placenta), chorion, amnion, allantois and yolk sac.
E8.5 left	E8.5	TS13: embryo	Present	5 µg; total RNA	ICR		Not Specified
E8.5 right	E8.5	TS13: extraembryonic component	Present	5 µg; total RNA	ICR		Not Specified	Extraembryonic tissues included ectoplacental cone (placenta), chorion, amnion, allantois and yolk sac.
E9.5 left	E9.5	TS15: embryo	Present	5 µg; total RNA	ICR		Not Specified
E9.5 right	E9.5	TS15: extraembryonic component	Present	5 µg; total RNA	ICR		Not Specified	Extraembryonic tissues included ectoplacental cone (placenta), chorion, amnion, allantois and yolk sac.
E10.5 left	E10.5	TS17: embryo	Present	5 µg; total RNA	ICR		Not Specified
E10.5 right	E10.5	TS17: extraembryonic component	Present	5 µg; total RNA	ICR		Not Specified	Extraembryonic tissues included ectoplacental cone (placenta), chorion, amnion, allantois and yolk sac.
E11.5 left	E11.5	TS19: embryo	Present	5 µg; total RNA	ICR		Not Specified
E11.5 right	E11.5	TS19: extraembryonic component	Present	5 µg; total RNA	ICR		Not Specified	Extraembryonic tissues included ectoplacental cone (placenta), chorion, amnion, allantois and yolk sac.
E12.5 left	E12.5	TS20: embryo	Present	5 µg; total RNA	ICR		Not Specified
E12.5 right	E12.5	TS20: extraembryonic component	Present	5 µg; total RNA	ICR		Not Specified	Extraembryonic tissues included ectoplacental cone (placenta), chorion, amnion, allantois and yolk sac.
E13.5 left	E13.5	TS21: embryo	Present	5 µg; total RNA	ICR		Not Specified
E13.5 right	E13.5	TS21: extraembryonic component	Present	5 µg; total RNA	ICR		Not Specified	Extraembryonic tissues included ectoplacental cone (placenta), chorion, amnion, allantois and yolk sac.
E14.5 left	E14.5	TS22: embryo	Present	5 µg; total RNA	ICR		Not Specified
E14.5 right	E14.5	TS22: extraembryonic component	Present	5 µg; total RNA	ICR		Not Specified	Extraembryonic tissues included ectoplacental cone (placenta), chorion, amnion, allantois and yolk sac.
E15.5 left	E15.5	TS23: embryo	Present	5 µg; total RNA	ICR		Not Specified
E15.5 right	E15.5	TS23: extraembryonic component	Present	5 µg; total RNA	ICR		Not Specified	Extraembryonic tissues included ectoplacental cone (placenta), chorion, amnion, allantois and yolk sac.
E16.5 left	E16.5	TS24: embryo	Present	5 µg; total RNA	ICR		Not Specified
E16.5 right	E16.5	TS24: extraembryonic component	Present	5 µg; total RNA	ICR		Not Specified	Extraembryonic tissues included ectoplacental cone (placenta), chorion, amnion, allantois and yolk sac.
E17.5 left	E17.5	TS25: embryo	Present	5 µg; total RNA	ICR		Not Specified
E17.5 right	E17.5	TS25: extraembryonic component	Present	5 µg; total RNA	ICR		Not Specified	Extraembryonic tissues included ectoplacental cone (placenta), chorion, amnion, allantois and yolk sac.
E18.5 left	E18.5	TS26: embryo	Present	5 µg; total RNA	ICR		Not Specified
E18.5 right	E18.5	TS26: extraembryonic component	Present	5 µg; total RNA	ICR		Not Specified	Extraembryonic tissues included ectoplacental cone (placenta), chorion, amnion, allantois and yolk sac.

Notes:
(a) Expression in embryos increased gradually until E13.5, then suddenly declined between E14.5 and E15.5 and sustained low levels until E18.5. Expression in extraembryonic tissues were higher than in embryos, except E12.5.

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