Summary | Mutation origin | Mutation description | Find Mice (IMSR) | References

Symbol:	Samd8tm2Kwi
Name:	sterile alpha motif domain containing 8; targeted mutation 2, Klaus Willecke
MGI ID:	MGI:5707958
Synonyms:	SMSrflox
Gene:	Samd8  Location: Chr14:21800599-21848794 bp, + strand  Genetic Position: Chr14, 11.86 cM, cytoband B
Alliance:	Samd8tm2Kwi page

Germline Transmission:	Earliest citation of germline transmission: J:228568
Parent Cell Line:	HM-1 (ES Cell)
Strain of Origin:	129P2/OlaHsd-Hprt1b-m3

Allele Type:    Targeted (Reporter) Mutation:    Insertion   Mutation details: The gene has been modified by insertion of a loxP site upstream of exon 5, a frt-flanked SV40-neo cassette followed by a second loxP site downstream of the 3' UTR, and a second copy of exon 5 followed in-frame by the EGFP coding sequence. Cre recombinase-mediated excision of the endogenous exon 5 through the 3' untranslated region will result in splicing of exon 4 to the downstream copy of exon 5, leading to production of a SAMD8/EGFP fusion protein lacking the catalytic domain encoded by exon 6. (J:228568)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Samd8 Mutation:	31 strains or lines available

Original:	J:228568 Bickert A, et al., Functional characterization of enzymes catalyzing ceramide phosphoethanolamine biosynthesis in mice. J Lipid Res. 2015 Apr;56(4):821-35
All:	1 reference(s)